Biotechnology Letters

, Volume 9, Issue 3, pp 163–168

Construction of a shuttle vector betweenEscherichiacoli andZymomonasanaerobia

  • Ki Hong Yoon
  • M. Y. Pack
Article

Summary

A 1.7-kb cryptic plasmid was isolated fromZymomonasanaerobia and used to construct a shuttle vector inserting useful parts of pUC9, pBR322, and pRK2501.Escherichiacoli was employed to clone the new plasmid designated pSR12. The 7.7-kb plasmid pSR12 reisolated from the host cells could transform competent cells ofZ.anaerobia at 2×10−7 frequency. This shuttle vector contains two antibiotic resistance markers, Kanr and Tetr, as well as restriction sites such as EcoRI, PstI, and XhoI, suitable for DNA recombinations.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Birnboim, H. C. and Doly, J. (1979). Nucleic Acid Res. 7, 1515–1523.Google Scholar
  2. Bolivar, F., Rodriquez, R. L., Greene, P. J., Betlach, M. C., Heyneker, H. L. and Boyer, H. W. (1977). Gene. 2, 95–113.Google Scholar
  3. Browne, G. M., Skonicki, M. L., Goodman, A. E. and Rogers, P. L. (1984). Plasmid. 12, 211–214.Google Scholar
  4. Carey, V. C., Walia, S. K. and Ingram, L. O. (1983). Appl. Environ. Microbiol. 46, 1163–1168.Google Scholar
  5. Dally, E. L., Stokes, H. W. and Eveleigh, D. E. (1982). Biotech. Letter. 4, 91–96.Google Scholar
  6. Goodman, H. M. and Macdonald, R. J. (1979). In: Methods in Enzymology, R. Wu, eds. vol. 68, pp. 75–90, New York: Academic Press.Google Scholar
  7. Kahn, M., Kolter, R., Thomas, C., Figurski, D., Meyer, R., Remaut, E. and Helinski, D. R. (1979). In: Methods in Enzymology, R. Wu, eds. vol. 68, pp. 268–280, New York: Academic Press.Google Scholar
  8. Maniatis, T., Fritsh, E. F. and Sambrook, J. (1982). In: Molecular Cloning — A Laboratory Manual, Cold Spring Harbor.Google Scholar
  9. McDonnell, M. W., Simon, M. N. and Studier, F. W. (1977). J. Mol. Biol. 110, 119–146.Google Scholar
  10. Skotnicki, M. L., Tribe, D. E. and Rogers, P. L. (1980). Appl. Environ. Microbiol. 40, 7–12.Google Scholar
  11. Stokes, H. W., Dally, E. L., Yablonsky, M. O. and Eveleigh, D. E. (1983). Plasmid. 9, 138–146.Google Scholar
  12. Tonomura, K., Okamoto, T., Yasui, M. and Yanase, H. (1986). Agric. Biol. Chem. 50, 805–808.Google Scholar
  13. Vieira, J. and Messing, J. (1982). Gene. 19, 259–268.Google Scholar

Copyright information

© Kluwer Academic Publishers 1987

Authors and Affiliations

  • Ki Hong Yoon
    • 1
  • M. Y. Pack
    • 1
  1. 1.Department of Biological Science and EngineeringKorea Advanced Institute of Science and TechnologySeoulKorea

Personalised recommendations