Determination of local myoglobin concentration in the guinea pig heart
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Summary
A method is described for calculating local myoglobin concentration in hemoglobin-free perfused guinea-pig heart by measuring the decrease of tissuePo2 during stop of perfusion. The myoglobin concentration in ca. 10 nanogram of tissue varies between 0.106 and 0.676 g-% of the wet weight. The mean value of 0.373 g-% (standard deviation of ±0.138) agrees very well with the values obtained with chemical methods by other authors. The great variation of our values is interpreted as an inhomogeneous distribution of the local myoglobin concentration.
Key words
Myoglobin Concentration Local Tissue RespirationPreview
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© Springer-Verlag 1973