Journal of Chemical Ecology

, Volume 14, Issue 1, pp 181–187 | Cite as

Bioassays for allelopathy: Measuring treatment responses with independent controls

  • G. Bruce Williamson
  • Donald Richardson
Article

Abstract

In bioassays for allelopathy, where responses to treatments are determined in conjunction with responses to independent controls, statistical comparisons among treatments require an index which measures each treatment response (T) in relation to its control response (C). The most commonly used index, the treatment-control ratio (T/C), exhibits two analytical problems. First, means ofT/C values are distorted upward when any of the individual values is greater than one, i.e., when stimulation occurs. Second, the distribution ofT/C values may not be normal and homoscedastic. We provide two alternative indices of response whose means do not exhibit the upward distortion ofT/C means. Then, the two indices are compared toT/C values in an empirical test for normality and homoscedasticity on a large bioassay data set. Results indicate that for this data set, one of the alternatives,RI, is clearly superior.RI is defined as 1 — (C/T) ifTC and asT/C — 1 ifT <C.

Key words

Bioassay response index allelopathy normality homoscedasticity parametric analysis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. AlSaadawi, I.S., andAlRubeaa, A.J. 1985. Allelopathic effects ofCitrus aurantium L. I. Vegetational patterning.J. Chem. Ecol. 11:1515–1525.Google Scholar
  2. AlSaadawi, I.S., Arif, M.B., andAlRubeaa, A.J. 1985. Allelopathic effects ofCitrus aurantium L. II. Isolation, characterization, and biological activities of phytotoxins.J. Chem. Ecol. 11:1527–1534.Google Scholar
  3. Beckenbach, E.F., andBellman, R. 1961. Inequalities. Springer-Verlag, Berlin.Google Scholar
  4. Carter, M.F., andGrace, J.B. 1986. Relative effects ofJusticia americana litter on germination, seedlings, and established plants ofPolygonum lapathifolium.Aquat. Bot. 23:341–349.Google Scholar
  5. Gilmore, A.R. 1985. Allelopathic effects of giant foxtail on germination and radicle elongation of loblolly pine seed.J. Chem. Ecol. 11:583–591.Google Scholar
  6. Newman, E.I. 1978. Allelopathy: Adaptation or accident, pp. 327–342,in J.B. Harborne (ed.). Biochemical Aspects of Plant and Animal Coevolution. Academic Press. London.Google Scholar
  7. Richardson, D.R. 1985. Allelopathic effects of species in the sand pine scrub of Florida. PhD dissertation. University of South Florida, Tampa.Google Scholar
  8. Sokal, R.R., andRohlf, F.J. 1969. Biometry. W.H. Freeman, San Francisco.Google Scholar
  9. Stevens, G.A., Jr., andTang, C.S. 1985. Inhibition of seedling growth of crop species by recirculating root exudates ofBidens pilosa L.J. Chem. Ecol. 11:1411–1425.Google Scholar

Copyright information

© Plenum Publishing Corporation 1988

Authors and Affiliations

  • G. Bruce Williamson
    • 1
  • Donald Richardson
    • 2
  1. 1.Department of BotanyLouisiana State University Baton RougeLouisiana
  2. 2.Department of BiologyUniversity of South Florida TampaFlorida

Personalised recommendations