Biotechnology Letters

, Volume 13, Issue 12, pp 889–892 | Cite as

Production of rosmarinic acid from perfusion culture ofAnchusa officinalis in a membrane-aerated bioreactor

  • Wei Wen Su
  • Arthur E. Humphrey


In this study, a perfusion fermentation ofAnchusa officinalis was carried out in a stirred tank bioreactor integrated with an internal cross-flow filter. Bubble-free aeration via microporous membrane fibers was used to provide oxygen. A two-stage culture was successfully conducted in this reactor without filter fouling. In a 17 day fermentation, a cell density of 26 g dw/I and a rosmarinic acid productivity of 94 mg/l day were achieved. This productivity is three times that obtained in a batch culture.


Oxygen Fermentation Organic Chemistry Cell Density Aeration 
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  2. Su, W. W. (1991). Production of plant secondary metabolites from high density perfusion cultures in a membrane aerated bioreactor. Ph.D. Thesis, Lehigh UniversityBethlehem.Google Scholar
  3. Su, W. W., Caram, H. S., and Humphrey, A. E. (1991). Optimal design of the tubular microporous membrane aerator for shear sensitive cell cultures. Accepted for publication inBiotechnology Progress.Google Scholar
  4. Su, W. W., and Humphrey, A. E. (1990). Production of rosmarinic acid from high density perfusion culture ofAnchusa officinalis using a high sugar medium.Biotechnology Letters, 12: 793–798.Google Scholar

Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Wei Wen Su
    • 1
  • Arthur E. Humphrey
    • 2
  1. 1.Department of Agricultural EngineeringUniversity of HawaiiHonoluluUSA
  2. 2.Department of Chemical EngineeringLehigh UniversityBethlehemUSA

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