Biotechnology Letters

, Volume 13, Issue 12, pp 863–866 | Cite as

Lithium chloride effect on phenylethyl-B-D-galactoside synthesis by Aspergillus oryzae B-D-galactosidase in the presence of high lactose concentration

  • Yannick Fortun
  • Bernard Colas
Article

Summary

Phenylethyl-β-D-galactoside (PEGal) synthesis by transglycosylation can be catalysed by Aspergillus oryzae β-galactosidase and was studied using high concentrations of glycosyl donor (lactose). The amount of PEGal formed reached a maximum value after 1Oh in the presence of 0.5M lactose. Beyond this time, PEGal was slowly hydrolysed. LiCl between 0.4M and 0.7M in the reaction medium prevented the progressive disappearance of the product.

Keywords

Chloride Lithium Organic Chemistry Lactose Aspergillus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Yannick Fortun
    • 1
  • Bernard Colas
    • 1
  1. 1.Laboratoire de biochimie II, Centre de Recherche de Biologie et Physico-chimie CellulairesFaculté des Sciences et des TechniquesNantes Cedex 03France

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