Biotechnology Letters

, Volume 16, Issue 2, pp 205–210 | Cite as

Ethanol production from concentrated whey permeate using a fed-batch culture ofKluyveromyces fragilis

  • Mario Daniel Ferrari
  • Lilián Loperena
  • Hermosinda Varela


Fed-batch fermentation of non-supplemented concentrated whey permeate resulted in high ethanol productivity for feeds of lactose for which batch fermentation had a poor performance. At an initial lactose concentration of 100 g/L and a constant lactose feeding rate of 18 g/h we have obtained: ethanol concentration 64 g/L, ethanol productivity 3.3 g/Lh, lactose consumption 100%, ethanol yield 0.47 g/g, and biomass yield 0.058 g/g.


Biomass Fermentation Organic Chemistry Lactose Bioorganic Chemistry 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



total lactose fed per medium volume in the bioreactor, g/L


initial lactose concentration, g/L


lactpse feeding rate, g/h


final ethanol concentration, g/L


ethanol yield, g ethanol/g lactose


biomass yield, g biomass/g lactose


lactose consumption, %


overall ethanol volumetric productivity, g/Lh


maximum specific growth rate, h


maximum specific lactose consumption rate, g/gh


maximum specific ethanol production rate, g/gh


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  1. Bothast, R. J., Kurtzman, C. P., Saltarelli, M. D., and Slininger (1986).Biotechnol. Lett. 8, 593–596.Google Scholar
  2. Chen, C., Dale, M. C., and Okos, M. R. (1990).Biotechnol. Bioeng. 36, 975–982.Google Scholar
  3. Guimaraes, W. V., Dudey, G. L., and Ingram, L. O. (1992).Biotechnol. Bioeng. 40, 41–45.Google Scholar
  4. Hahn-Hägerdal, B. (1985). Biotechnol. Bioeng. 27, 914–916.Google Scholar
  5. Jones, T. D., Havard, J. M., and A. J. Daugulis (1993).Biotechnol. Lett. 15, pp. 871–876.Google Scholar
  6. Maiorella, B. L. and Castillo, F. J. (1984).Process Biochem. 19, 117–161.Google Scholar
  7. Marwaha, S. S., Kennedy, J. F., and Sehgal, V. K., (1988).Process Biochem. 23, 17–22.Google Scholar
  8. Mawson, A. J. (1987).Australia J. Biotechnol. 1, 71–74.Google Scholar
  9. Mawson, A. J. (1988).Biotechnol. Lett. 10 503–508.Google Scholar
  10. Murtagh, J. F. (1986). The Production of Ethanol from Cheese Whey in Ireland, New Zealand, and The United States of America Using the Carberry Process. In:VII International Symposium on Fuel Alcohols, pp. 115–120. Paris: Editions Technip.Google Scholar
  11. Tin, C. S. F. and A. J. Mawson (1993).Process Biochem. 38, 217–221.Google Scholar
  12. Tu, C. W., Jayanata, Y., and Bajpai, R. (1985).Biotechnol. Bioeng. Symp. 15, 295–305.Google Scholar
  13. Varela, H., Ferrari, M. D., Loperena, L., and Lareo, C. (1992).Microbiologia SEM 8, 14–20.Google Scholar
  14. Vienne, P. and von Stockar, U. (1985).Biotechnol. Lett. 7, 521–526.Google Scholar
  15. Vienne, P. and von Stockar, U. (1985).Enzyme Microb. Technol. 7, 287–294.Google Scholar
  16. Zertuche, L. and Zall, R. R. (1985).Biotechnol. Bioeng. 27, 547–554.Google Scholar

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Mario Daniel Ferrari
    • 1
  • Lilián Loperena
    • 1
  • Hermosinda Varela
    • 1
  1. 1.Depto. Bioingeniería, Instituto de Ingeniería Química Facultad de IngenieríaUniversidad de la RepúblicaMonievideoURUGUAY

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