Biotechnology Letters

, Volume 14, Issue 8, pp 639–642 | Cite as

Production of HIV-1 Vpu with pYEULCBX, a convenient vector for the production of non-fused proteins in yeast

  • I. G. Macreadie
  • P. Failla
  • O. Horaitis
  • A. A. Azad
Article

Summary

Site directed mutagenesis was employed to modify a vector previously constructed for the convenient, copper-inducible production of fusion proteins in the yeastSaccharomyces cerevisiae. The resulting vector, pYEULCBX, remains versatile and now permits nonfused proteins to be produced. Thevpu gene from HIV-1 was expressed as an example.

Keywords

Organic Chemistry Fusion Protein Bioorganic Chemistry Site Directed Mutagenesis Convenient Vector 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • I. G. Macreadie
    • 1
  • P. Failla
    • 1
  • O. Horaitis
    • 1
  • A. A. Azad
    • 1
  1. 1.Biomolecular Research InstituteParkvilleAustralia

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