Biotechnology Letters

, Volume 8, Issue 11, pp 771–776

Synthesis of eicosapentaenoyl phosphatidylcholines by polyethylene glycol-modified lipase in benzene

  • Takayuki Yoshimoto
  • Masahide Nakata
  • Shigehiko Yamaguchi
  • Tadashi Funada
  • Yuji Saito
  • Yuji Inada
Article

Summary

Lipase fromCandida cylindracea was modified with activated polyethylene glycol, which was synthesized from α-carboxymethyl-ω-methoxypoly-(oxyethylene) and N-hydroxysuccinimide with dicyclohexylcarbodiimide. The modified lipase was soluble in organic solvents such as benzene and catalyzed the reaction of ester-exchange to synthesize eicosapentaenoyl phosphatidylcholines from dipalmitoyl phosphatidylcholine and eicosapentaenoic acid. Either one of palmitic acid at C-1 or C-2 position of the phosphatidylcholine was exchanged with eicosapentaenoic acid.

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Copyright information

© Science and Technology Letters 1986

Authors and Affiliations

  • Takayuki Yoshimoto
    • 1
  • Masahide Nakata
    • 1
  • Shigehiko Yamaguchi
    • 1
  • Tadashi Funada
    • 1
  • Yuji Saito
    • 1
  • Yuji Inada
    • 1
  1. 1.Laboratory of Biological ChemistryTokyo Institute of TechnologyTokyo 152Japan

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