Biotechnology Letters

, Volume 14, Issue 10, pp 937–940 | Cite as

Lipase-catalyzed hydrolysis of Crambe oil in AOT-isooctane reversed micelles

  • Johannes T. P. Derksen
  • F. Petrus Cuperus
Article

Summary

The hydrolysis by 1,3-specific lipases (Humicola lanuginosa, Mucor miehei, Rhizopus delemar andRhizopus javanicus) of the highly symmetric, high molecular weight triglycerides fromCrambe abyssinica (Crambe) seed oil is studied in an AOT-stabilized microemulsion system. Enzyme kinetic data shows that, of the lipases studied,Rhizopus javanicus lipases exerts the highest hydrolytic activity towards this new seed oil.

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References

  1. 1.
    Buhler, M., Wandrey, C. (1992),Fat Sci. Technol. 94, 82–94.Google Scholar
  2. 2.
    Han, D. and Rhee, J.S. (1986),Biotechnol. Bioeng. 28, 1250–1255.Google Scholar
  3. 3.
    Han, D., Kwon, D.Y. and Rhee, J.S. (1987),Agric. Biol. Chem. 51, 615–618.Google Scholar
  4. 4.
    Holmberg, K. and Osterberg, E. (1990).Progr. Colloid Polym. Sci. 82, 181–189.Google Scholar
  5. 5.
    Kierkels, J.G.T., Vleugels, L.F.W., Kem, J.H.A., Meijer, E.M. and Kloosterman, M. (1990).Enzyme Microb. Technol. 12, 760–763.Google Scholar
  6. 6.
    Kim, T. and Chung, K. (1989).Enzyme Microb. Technol. 11, 528–532.Google Scholar
  7. 7.
    Martinek, K., Levashov, A.V., Klyachko, N.L., Pantin, V.I. and Berezin, I.V. (1981).Biochim. Biophys. Acta 657, 277–294.Google Scholar
  8. 8.
    Martinek, K., Levashov, A.V., Klyachko, N., Khmelnitski, Y.L. and Berezin, I.V. (1986).Eur. J. Biochem. 155, 453–468.Google Scholar
  9. 9.
    Muuse, B.G., Cuperus, F.P. and Derksen, J.T.P., 1992. Composition and properties of oils from new oilseed crops.Ind. Crops Prod. 1, [in press]Google Scholar
  10. 10.
    Rattray, J., Casey, J. and Macrae, A. (1992).INFORM 3, 203–207.Google Scholar
  11. 11.
    Sonntag, N.O.V. (1991).INFORM 2, 449–463.Google Scholar
  12. 12.
    Tsai, S.-W. and Chiang, C.-L. (1991).Biotechnol. Bioengin. 38, 206–211.Google Scholar
  13. 13.
    Verger, R. and De Haas, G.H. (1976).Annu. Rev. Biophys. Bioeng. 5, 77–117.Google Scholar
  14. 14.
    Verger, R. (1980).Meth. Enzymol. 64, 340–392.Google Scholar
  15. 15.
    Walde, P., Giuliani, A.M., Boicelli, C.A. and Luisi, P.L. (1990).Chem. Phys. Lipids 53, 265–288.Google Scholar

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Johannes T. P. Derksen
    • 1
  • F. Petrus Cuperus
    • 1
  1. 1.Agrotechnological Research Institute (ATO-DLO)WageningenThe Netherlands

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