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Journal of Chemical Ecology

, Volume 13, Issue 1, pp 47–70 | Cite as

Cardenolide content and thin-layer chromatography profiles of monarch butterflies,danaus plexippus L., and their larval host-plant milkweed,asclepias viridis walt., in northwestern louisiana

  • Steven P. Lynch
  • Ronald A. Martin
Article

Abstract

This paper is the first in a series on cardenolide fingerprinting of monarch butterflies and their host-plant milkweeds in the eastern United States. Spectrophotometric determinations of the gross cardenolide content of 60Asclepias viridis plants in northwestern Louisiana indicate a positively skewed variation ranging from 95 to 432 υg/0.1 g dry weight with a mean of 245 υg/0.1 g. Butterflies reared individually on these plants contained a normal cardenolide distribution ranging from 73 to 591 υg/0.1 g dry weight with a mean of 337 υg/0.1 g. The uptake of cardenolide by the butterflies best fit a logarithmic function of the plant concentration. Female monarchs (385 υg/0.l g) contained significantly greater mean cardenolide concentrations than did males (287 υg/0.1 g). No indications of a metabolic cost for either cardenolide ingestion or storage were adduced from size or dry weight data. Thin-layer chromatograms of 24 individual plant-butterfly pairs developed in two solvent systems resolved 21 individual spots in the plants and 15 in the butterflies.A. viridis plants appear to contain several relatively nonpolar cardenolides of the calotropagenin series which are metabolized to the more polar 3'-hydroxy derivatives calactin and calotropin as well as to calotropagenin in the butterflies. The epoxy cardenolides labriformin and labriformidin were absent, although desglucosyrioside (a 3'-hydroxy derivative) appeared present in both plants and butterflies. Quantitative evaluation of theRf values, spot intensities, and probabilities of occurrence in the chloroform-methanol—formamide TLC system produced a cardenolide fingerprint clearly distinct from those previously established for monarchs reared on otherAsclepias species, supporting the use of fingerprints to make ecological predictions concerning larval host-plant utilization.A. viridis is the predominant early spring milkweed throughout most of the south central United States and may be important in providing chemical protection to spring and early summer generation monarchs in the eastern United States.

Key words

Asclepias viridis milkweed Asclepiadaceae Danaus plexippus Lepidoptera Danaidae monarch butterfly cardenolide cardiac glyco-side gitoxin emical ecology emical defense in-layer chromatog-raphy ant-insect interactions evolution 

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References

  1. Blum, M.S. 1981.Chemical Defense of Arthropods. Academic Press, New York. 562 pp.Google Scholar
  2. Brower, L.P. 1961. Studies on the migration of the monarch butterfly I. Breeding populations ofDanausplexippus andD. gilippus berenice in south central Florida.Ecology 42:76–83.Google Scholar
  3. Brower, L.P. 1962. Evidence for interspecific competition in natural populations of the monarch and queen butterflies,Danaus plexippus, andD. gillippus berenice in south central Florida.Ecology 43:549–552.Google Scholar
  4. Brower, L.P., 1977. Monarch migration.Nat. Hist. 86:40–53.Google Scholar
  5. Brower, L.P. 1984. Chemical defence in butterflies,in R.I. Vane-Wright and P.R. Ackery (eds.). The Biology of Butterflies. Academic Press, London, pp. 109–134.Google Scholar
  6. Brower, L.P., andGlazier, S.C. 1975. Localization of heart poisons in the monarch butterfly.Science 188:19–25.Google Scholar
  7. Brower, L.P., andMoffitt, C.M. 1974. Palatability dynamics of cardenolides in the monarch butterfly.Nature 249:280–283.Google Scholar
  8. Brower, L.P., McEvoy, P.B., Williams, K.L., andFlannery, M.A. 1972. Variation in cardiac glycoside content of monarch butterflies from natural populations in eastern North America.Science 177:426–429.Google Scholar
  9. Brower, L.P., Edmunds, M., andMoffitt, C.M. 1975. Cardenolide content and palatability of a population ofDanaus chrysippus butterflies from West Africa.J. Entomol. 49:183–196.Google Scholar
  10. Brower, L.P., Calvert, W.H., Hedrich, L.E., andChristian, J. 1977. Biological observations on an overwintering colony of monarch butterflies (Danaus plexippus Danaidae) in Mexico.J. Lepid. Soc. 31:232–242.Google Scholar
  11. Brower, L.P., Seiber, J.N., Roeske, C.N., andLynch, S.P. 1982. Plant-determined variation in the cardenolide content, thin-layer chromatography profiles, and emetic potency of monarch butterflies,Danaus plexippus L., reared on milkweed,Asclepias eriocarpa, in California.J. Chem. Ecol. 8:579–633.Google Scholar
  12. Brower, L.P., Seiber, J.N., Nelson, C.J., Lynch, S.P., Hoggard, M.P., andCohen, J.A. 1984a. Plant-determined variation in the cardenolide content, thin-layer chromatography profiles, and emetic potency of monarch butterflies,Danaus plexippus, reared on the milkweed,Asclepias californica, in California.J. Chem. Ecol. 10:1823–1857.Google Scholar
  13. Brower, L.P., Seiber, J.N., Nelson, C.J., Lynch, S.P., andHolland, M.M. 1984b. Plant-determined variation in the cardenolide content, thin-layer chromatography profiles, and emetic potency of monarch butterflies,Danaus plexippus, reared on the milkweed,Asclepias speciosa, in California.J. Chem. Ecol. 10:601–639.Google Scholar
  14. Bruschweiler, F., Stockel, K., andReichstein, T. 1969. Calotropis-Glykoside, vermutliche Teilstrukture.Helv. Chim. Acta 52:2276–2303.Google Scholar
  15. Calvert, W.H., andBrower, L.P. 1982. The importance of forest cover for the survival of overwintering monarch butterflies (Danaus plexippus, Danaidae).J. Lepid. Soc. 35:216–225.Google Scholar
  16. Calvert, W.H., Hedrick, L.E., andBrower, L.P. 1979. Mortality of the monarch butterfly (Danaus plexippus L.): Avian predation at five overwintering sites in Mexico.Science 204:847–850.Google Scholar
  17. Calvert, W.H., Zuchowski, W., andBrower, L.P. 1982. The impact of forest thinning on microclimate in monarch butterfly (Danaus plexippus L.) overwintering areas of Mexico.Bol. Soc. Bot. Mex. 42:11–18.Google Scholar
  18. Cohen, J.A. 1985. Differences and similarities in cardenolide contents of queen and monarch butterflies in Florida and their ecological and evolutionary implications.J. Chem. Ecol. 11:85–103.Google Scholar
  19. Cohen, J.A., andBrower, L.P. 1982. Oviposition and larval success of wild monarch butterflies (Lepidoptera: Danaidae) in relation to host plant size and cardenolide concentration.J. Kans. Entomol. Soc. 55(2):343–348.Google Scholar
  20. Dixon, C.A., Erickson, J.M., Kellett, D.N., andRothschild, M. 1978. Some adaptations betweenDanaus plexippus and its food plant, with notes onD. chrysippus andEuploea core (Insecta: Lepidoptera).J. Zool. London 185:437–467.Google Scholar
  21. Duffey, S.S. 1977. Arthropod allomones: chemical effronteries and antagonists.Proc. Int. Congr. Entomol. 15:323–394.Google Scholar
  22. Duffey, S.S, Blum, M.S., Isman, M.B., andScudder, O.G.E. 1978. Cardiac glycosides: A physical system for their sequestration by the milkweed bug.J. Insect Physiol. 24:639–645.Google Scholar
  23. Erickson, J.M. 1973. The utilization of variousAsclepias species by larvae of the monarch butterflyDanaus plexippus.Psyche 80:230–244.Google Scholar
  24. Fink, L.S., andBrower, L.P. 1981. Birds can overcome the cardenolide defence of monarch butterflies in Mexico.Nature 291:67–70.Google Scholar
  25. Isman, M.B., Duffey, S.S., andScudder, G.G.E.1977. Cardenolide content of some leaf-and stem-feeding insects on temperature North American milkweeds (Asclepias spp.).Can. J. Zool. 55:1024–1028.Google Scholar
  26. Marty, M.A., andKrieger, R.I. 1984. Metabolism of uscharidin, a milkweed cardenolide, by tissue homogenates of monarch butterfly larvae,Danaus plexippus L.J. Chem. Ecol. 10:945–956.Google Scholar
  27. Nelson, C.J., Seiber, J.N., andBrower, L.P. 1981. Seasonal and intraplant variation of cardenolide content in the California milkweed,Asclepias eriocarpa, and implications for plant defense.J. Chem. Ecol. 7:981–1010.Google Scholar
  28. Roeske, C.N., Seiber, J.N., Brower, L.P. andMoffitt, C.M. 1976. Milkweed cardenolides and their comparative processing by monarch butterflies (Danaus plexippus L.)Recent Adv. Phytochem. 10:93–167.Google Scholar
  29. SAS. 1982.SAS User's Guide: Statistics. SAS Institute Inc. Gary, North Carolina. 584 pp.Google Scholar
  30. Seiber, J.N., Tuskes, P.M., Brower, L.P. andNelson, C.J. 1980. Pharmacodynamics of some individual milkweed cardenolides fed to larvae of the monarch butterfly (Danaus plexippus L.).J. Chem. Ecol. 6:321–339.Google Scholar
  31. Seiber, J.N., Lee, S.M. andBenson, J.M. 1983. Cardiac glycosides (cardenolides) in species ofAsclepias (Asclepiadaceae),in R. F. Keeler and A.T. Tu (eds.). Handbook of Natural Toxins, Vol. 1. Plant and Fungal Toxins. Marcel Dekker, New York, pp. 43–83.Google Scholar
  32. Seiber, J.N., Lee, S.M., andBenson, J.M. 1984. Chemical characteristics and ecological significance of cardenolides isAsclepias (milkweed) species,in W.D. Nes, G. Fuller, and L. Tsai (eds.). Isopentenoids in plants: Biochemistry and Function. Marcel Dekker, New York, pp. 563–588.Google Scholar
  33. Steel, R.G.D., andTorrfe, J.H. 1960. Principles and Procedures of Statistics. McGraw-Hill, New York, xvi + 481 pp.Google Scholar
  34. Urquhart, F.A. 1960. The Monarch Butterfly. University of Toronto Press, Toronto, Canada. 361 pp.Google Scholar
  35. Urquhart, F.A. andUrquhart, N. R. 1976. The overwintering site of the eastern population of the monarch butterfly (Danaus p. plexippus; Danaidae) in south Mexico.J. Lepid. Soc. 30:153–158.Google Scholar
  36. Urquhart, F.A., andUrquhart, N.R. 1978. Autumnal migration routes of the eastern population of the monarch butterfly (Danaus plexippus L.:Danaidae:Lepidoptera) in North America to the overwintering site in the neovolcanic plateau of Mexico.Can. J. Zool. 56:1759–1764.Google Scholar
  37. Woodson, R.E., Jr. 1954. The North American speciesof Asclepias L.Ann. Mo. Bot. Gard. 41:1–211.Google Scholar

Copyright information

© Plenum Publishing Corporation 1987

Authors and Affiliations

  • Steven P. Lynch
    • 1
  • Ronald A. Martin
    • 2
  1. 1.Department of Biological SciencesLouisiana State UniversityShreveport
  2. 2.Department of ChemistryLouisiana State UniversityShreveport

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