Journal of Chemical Ecology

, Volume 14, Issue 2, pp 453–461 | Cite as

Extraction of tannin from fresh and preserved leaves

  • Ann E. Hagerman


The extractability of tannin from fresh, lyophilized, and dried leaves collected at various times in the growing season was determined using the radial diffusion assay for protein-precipitating phenolics. The amount of tannin extracted depended on the method of leaf preservation and on the maturity of the leaf. Early in the season, more tannin was extracted from lyophilized leaves than from fresh leaves, but late in the season more tannin was extracted from fresh leaves. At all times, more tannin was extracted with aqueous acetone than with aqueous or acidic methanol.

Key words

Leaf preservation tannin extraction phenolic analysis protein precipitation 


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Copyright information

© Plenum Publishing Corporation 1988

Authors and Affiliations

  • Ann E. Hagerman
    • 1
  1. 1.Department of ChemistryMiami UniversityOxford

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