Journal of Chemical Ecology

, Volume 14, Issue 10, pp 1941–1950 | Cite as

Bioassays of segregating plants

A strategy for studying chemical defenses
  • S. L. Sinden
  • L. L. Sanford
  • W. W. Cantelo
  • K. L. Deahl
Article

Abstract

Solanum chacoense is a wild potato species resistant to the Colorado potato beetle,Leptinotarsa decemlineata. Most genotypes ofS. chacoense synthesize the glycoalkaloids solanine (sol) and chaconine (chac) and are hosts of the beetle. A few rare genotypes have a gene(s) for acetylation of carbon-23 of the steroid aglycone of sol and chac. Laboratory bioassays and replicated field tests of clones differing in the presence or absence of the acetyl moiety showed that acetylation of sol and chac markedly affects the response of both adults and larvae to the foliage. Adult feeding deterrency conferred by acetylated forms of sol and chac (leptines) in leaf-disk preference tests was consistent with the degree of antixenosis measured in the field. Development of larvae on foliage of clones with leptines was also inhibited. The studies support the validity of using laboratory bioassays of plants segregating for levels of a suspected defense compound to determine the role the compound has in defending the plant from attack by an insect predator in the field.

Key words

Glycoalkaloids insect resistance Colorado potato beetle leptines solanine chaconine Leptinotarsa decemlineata Solanum chacoense Solanum tuberosum 

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References

  1. Bongers, W. 1970. Aspects of host-plant relationship of the Colorado beetle.Meded. Landbouwhogesch. Wageningen. 70:1–77.Google Scholar
  2. Cantelo, W.W., Douglass, L.W., Sanford, L.L., Sinden, S.L., andDeahl, K.L. 1987. Measuring resistance to the Colorado potato beetle in potato.J. Entomol. Sci. 22:245–252.Google Scholar
  3. Dimock, M.B., andTingey, W.M. 1985. Resistance inSolanum spp. to the Colorado potato beetle: Mechanisms, genetic resources, and potential, pp. 79–106,in D.N. Ferro and R.H. Voss (eds.). Proc. Symp. on the Colorado potato beetle, XVIIth Int. Cong. Entomol., Mass. Agric. Exp. Sta. Bull. 704.Google Scholar
  4. Dimock, M.B., LaPointe, S.L., andTingey, W.M. 1986.Solanum neocardenasii: A new source of potato resistance to the Colorado potato beetle.J. Econ. Entomol. 79:1269–1275.Google Scholar
  5. Hsiao, T.H. 1974. Chemical influence on feeding behavior ofLeptinotarsa beetles, pp. 239–248,in L. Barton Browne (ed.). Experimental Analysis of Insect Behaviour. Springer-Verlag, Berlin.Google Scholar
  6. Kuhn, R., andLow, I. 1961. Zur Konsitution des Leptins.Chem. Ber. 94:1088–1095.Google Scholar
  7. McCollum, G.D., andSinden, S.L. 1978. Inheritance study of tuber glycoalkaloids inSolanum chacoense.Am. Potato J. 56:95–113.Google Scholar
  8. Mitchell, B.K., andHarrison, G.D. 1985. Effects ofSolanum glycoalkaloids on chemosensilla in the Colorado potato beetle. A mechanism of feeding deterrence?J. Chem. Ecol. 11:73–83.Google Scholar
  9. Osman, S.F., Sinden, S.L., Deahl, K., andMoreau, R. 1987. Metabolism of solanidine by microsomal fractions fromSolanum chacoense.Phytochemistry 26:3163–3165.Google Scholar
  10. Rosenthal, G.A. 1986. The chemical defenses of higher plants.Sci. Am. 254:94–99.Google Scholar
  11. Sanford, L.L., andSinden, S.L. 1972. Inheritance of potato glycoalkaloids.Am. Potato J. 49:209–217.Google Scholar
  12. Schalk, J.M., andStoner, A.K. 1976. A bioassay differentiates resistance to the Colorado potato beetle on tomatoes.J. Am. Soc. Hortic. Sci. 101:74–76.Google Scholar
  13. Schreiber, K. 1968. Steroid alkaloids: TheSolanum group, pp. 1–192,in R.H.F, Manske (ed.). The Alkaloids. Academic Press, New York.Google Scholar
  14. Sinden, S.L., Sanford, L.L., andOsman, S.F. 1980. Glycoalkaloids and resistance to the Colorado potato beetle inSolanum chacoense Bitt.Am. Potato J. 57:331–343.Google Scholar
  15. Sinden, S.L., Sanford, L.L., andDeahl, K.L. 1986a. Segregation of leptine glycoalkaloids inSolanum chacoense Bitter.J. Agric. Food Chem. 34:372–377.Google Scholar
  16. Sinden, S.L., Sanford, L.L., Cantelo, W.W., andDeahl, K.L. 1986b. Leptine glycoalkaloids and resistance to the Colorado potato beetle (Coleoptera: Chrysomelidae) inSolanum chacoense.Environ. Entomol. 15:1057–1062.Google Scholar
  17. Sturckow, B., andLow, I. 1961. Die Wirkung einiger Solanum-Alkaloidglykoside auf den Kartoffelkafer,Leptinotarsa decemlineata Say.Entomol. Exp. Appl. 4:133–142.Google Scholar
  18. Tingey, W.M. 1984. Glycoalkaloids as pest resistance factors.Am. Potato J. 61:157–167.Google Scholar
  19. Torka, M. 1950. Breeding potatoes with resistance to the Colorado potato beetle.Am. Potato J. 27:263–271.Google Scholar

Copyright information

© Plenum Publishing Corporation 1988

Authors and Affiliations

  • S. L. Sinden
    • 1
  • L. L. Sanford
    • 1
  • W. W. Cantelo
    • 1
  • K. L. Deahl
    • 1
  1. 1.U.S. Department of AgricultureVegetable Laboratory, Agriculture Research ServiceBeltsville

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