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Journal of Chemical Ecology

, Volume 11, Issue 11, pp 1535–1544 | Cite as

Use of dye-labeled protein as spectrophotometric assay for protein precipitants such as tannin

  • Thomas N. Asquith
  • Larry G. Butler
Article
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Accepted:

Abstract

Bovine serum albumin has been covalently labeled with Remazol brilliant blue R to provide a substrate for a convenient spectrophotometric assay for protein precipitants. The blue protein is especially useful for measuring protein precipitation by vegetable tannins because its absorption maximum is at a wavelength where plant pigments exhibit minimum absorption. Blue BSA has been used to determine, by competition experiments, the relative affinity of various proteins for tannins. A procedure for purifying condensed tannin from commercially available quebracho extract is described.

Key words

Tannin assay protein precipitation sorghum tannin quebracho tannin condensed tannins 
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Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • Thomas N. Asquith
    • 1
  • Larry G. Butler
    • 1
  1. 1.Department of BiochemistryPurdue UniversityWest Lafayette

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