Journal of Chemical Ecology

, Volume 15, Issue 9, pp 2347–2355

Induction of cytochrome P450-mediated detoxification of xanthotoxin in the black swallowtail

  • Michael B. Cohen
  • May R. Berenbaum
  • Mary A. Schuler
Article
  • 144 Downloads

Abstract

Xanthotoxin is a phototoxic allomone found in many of the host plants of the black swallowtail,Papilio polyxenes (Lepidoptera: Papilionidae). When added to the diet of final instar larvae, xanthotoxin can induce the cytochrome P450 monooxygenase (P450) activity in midgut microsomes by which it is detoxified. Induction is dose-dependent, increasing sevenfold when larvae feed on parsley treated topically with xanthotoxin at 0.5 or 1.0% fresh weight. Although xanthotoxin exerts much of its toxic effects when photoactivated by ultraviolet light, induction of P450 activity did not differ in the presence or absence of ultraviolet light. Despite a 4.7-fold induction of xanthotoxin-metabolizing P450 activity, total P450 content measured in the same microsomal samples did not increase significantly. These data indicate that multiple forms of P450 exist in the black swallowtail midgut and that they are differentially induced by xanthotoxin.

Key words

Black swallowtail Papilio polyxenes Lepidoptera Papilionidae microsomal cytochrome P450 induction xanthotoxin furanocoumarins plant defensive compounds 

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Copyright information

© Plenum Publishing Corporation 1989

Authors and Affiliations

  • Michael B. Cohen
    • 1
  • May R. Berenbaum
    • 1
  • Mary A. Schuler
    • 2
  1. 1.Department of EntomologyUniversity of IllinoisUrbana
  2. 2.Department of Plant BiologyUniversity of IllinoisUrbana

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