The Histochemical Journal

, Volume 4, Issue 6, pp 505–515 | Cite as

Bromine-Sudan Black: a general stain for lipids including free cholesterol

  • O. B. Bayliss
  • C. W. M. Adams


Preliminary bromination (with bromine water) increases the intensity of staining of tissue lipids with Sudan Black and certain other dyes. The mechanism appears to be due to the formation of sudanophilic bromo-derivatives of cholesterol and to the retention of certain other lipids, notably phosphatidyl choline and free fatty acids, during staining. The advantage of the bromine-Sudan Black method is that all tissue lipids are stained, except saturated fatty acids, saturated triglycerides and perhaps saturated cholesterol esters. In practice, such lipids rarely, if ever, occur alone, and normally are admixed with their stainable unsaturated counterparts.


Lipid Cholesterol Ester Triglyceride Free Fatty Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Chapman and Hall Ltd 1972

Authors and Affiliations

  • O. B. Bayliss
    • 1
  • C. W. M. Adams
    • 1
  1. 1.Department of PathologyGuy's Hospital Medical SchoolLondon

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