Visualization of retinal and choroidal blood flow with fluorescein leukocyte angiography in rabbits

  • Yunsik Yang
  • Sangduck Kim
  • Jaeduck Kim
Laboratory Investigation

Abstract

• Purpose: To visualize the retinal and choroidal leukocytes in rabbits with a new technique, fluorescein leukocyte angiography using a scanning laser ophthalmoscope. • Methods: Blood was withdrawn from an ear vein of a rabbit (New Zealand White), mixed with fluorescein dye in a test tube and centrifuged. The yellow-brown coat layer containing fluorescein-stained leukocytes was collected and injected into the ear vein of the same rabbit while performing fluorescein angiography with a scanning laser ophthalmoscope. The angiographic image displaying circulating fluorescent leukocytes in retinal and choroidal vessels was recorded on a videotape. • Results: Fluorescent leukocytes were clearly visible in the retinal arteries, capillaries, veins and choroidal vessels for more than 1 h. Plugging of leukocytes was seen throughout this period of time in choroidal vessels, while plugging was rare in retinal vessels. • Conclusions: Fluorescein leukocyte angiography is a new technique which can be used for visualization of the leukocytes in retinal and choroidal vessels non-invasively and in vivo.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Arend O, Harris A, Sponsel WE, Remky A, Reim M, Wolf S (1995) Macular capillary particle velocities: a blue field and scanning laser comparison. Graefe's Arch Clin Exp Ophthalmol 233:244–249Google Scholar
  2. 2.
    Engler RL, Schmid-Schönbein GW, Pavelec RS (1983) Leukocyte capillary plugging in myocardial ischemia and reperfusion in the dog. Am J Pathol 111:98–111Google Scholar
  3. 3.
    Hatchell DL, Wilson CA, Saloupis P (1994) Neutrophils plug capillaries in acute experimental retinal ischemia. Microvasc Res 47:344–354Google Scholar
  4. 4.
    Jung F, Kiesewetter H, Körber N, Wolf S, Reim M, Müller G (1983) Quantification of characteristic bloodflow parameters in the vessels of the retina with a picture analysis system for video-fluorescence angiograms: initial findings. Graefe's Arch Clin Exp Ophthalmol 221:133–136Google Scholar
  5. 5.
    Khoobehi B, Peyman GA (1994) Fluorescent vesicle system (a new technique for measuring blood flow in the retina). Ophthalmology 101:1716–1726Google Scholar
  6. 6.
    Khoobehi B, Niesman MR, Peyman GA, Oncel M (1989) Repetitive, selective angiography of individual vessels of the retina. Retina 9:87–96Google Scholar
  7. 7.
    Khoobehi B, Peyman GA, Vo K (1992) Laser triggered repetitive fluorescein angiography. Ophthalmology 99:72–79Google Scholar
  8. 8.
    Kimura H, Kiryu J, Nishiwaki H, Ogura Y (1995) A new fluorescent imaging procedure in vivo for evaluation of the retinal microcirculation in rats. Curr Eye Res 14:223–228Google Scholar
  9. 9.
    Kiryu J, Shahidi M, Mori MT, Ogura Y, Asrani S, Zeimer R (1994) Noninvasive visualization of the choriocapillaris and its dynamic filling. Invest Ophthalmol Vis Sci 35:3724–3731Google Scholar
  10. 10.
    Koyama T, Matsuo N, Shimizu K, Mihara M, Tsuchida Y, Wolf S, Reim M (1990) Retinal circulation timed in quantitative fluorescein angiography. Graefe's Arch Clin Exp Ophthalmol 228:442–446Google Scholar
  11. 11.
    Nasemann JE, Müller M (1990) Scanning laser angiography. In: Nasemann JE, Burk ROW (eds) Scanning laser ophthalmoscopy and tomography. Quintessenz, Munich, pp 63–80Google Scholar
  12. 12.
    Nishiwaki H, Ogura Y, Kimura H, Kiryu J, Honda Y (1995) Quantitative evaluation of leukocyte dynamics in retinal microcirculation. Invest Ophthalmol Vis Sci 36:123–130Google Scholar
  13. 13.
    Ohnishi Y, Fujisawa K, Ishibashi T, Kojima H (1994) Capillary blood flow velocity measurements in cystoid macular edema with the scanning laser ophthalmoscope. Am J Ophthalmol 17:24–29Google Scholar
  14. 14.
    Prëußner PR, Richard G, Darrelmann O, Weber J, Kreissig I (1983) Quantitative measurement of retinal blood flow in human beings by application of digital image-processing methods to television fluorescein angiograms. Graefe's Arch Clin Exp Ophthalmol 221:110–112Google Scholar
  15. 15.
    Riva CE, Feke GT, Ben-Sira I (1978) Fluorescein dye-dilution technique and retinal circulation. Am J Physiol 234:H315-H322Google Scholar

Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • Yunsik Yang
    • 1
  • Sangduck Kim
    • 1
  • Jaeduck Kim
    • 1
  1. 1.Department of Ophthalmology, School of MedicineWonkwang UniversityIksan, ChonbukSouth Korea

Personalised recommendations