The Histochemical Journal

, Volume 21, Issue 3, pp 172–178 | Cite as

Immunolocalization of collagen types I and III in the arterial wall of the rat

  • Colin Farquharson
  • Simon P. Robins
Papers
Received:
Revised:

Summary

Collagen types I and III were located by immunofluorescence procedures in the aorta and coronary arteries of the rat. Type I collagen was most prevalent in the adventitia of the aorta with only small amounts present in the intima and media. Type III collagen appeared to be a significant component in the media of the aorta and also in the adventitia of both blood vessels. The intima and media of the coronary arteries did not stain strongly for either type I or III collagen. Neither staining procedure was altered with preincubation of the sections with hyaluronidase or chondroitinase ABC. These studies indicate that type III collagen is a major component of the adventitia which has previously not been recognized by immunohistochemical techniques, possibly due to masking of collagen staining with glycosaminoglycans.

Keywords

Collagen Coronary Artery Blood Vessel Collagen Type Arterial Wall 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Barnard, K., Partridge, S. M., Whiting, A. H., Fantl, V. &McCullagh, K. G. (1982) Immunological studies with human aortic elastin.Connect. Tissue Res 9, 233–40.Google Scholar
  2. Bartholomew, J. S. &Anderson, J. C. (1983) Investigation of relationships between collagens, elastin and proteoglycans in bovine thoracic aorta by immunofluorescence techniques.Histochem. J. 15, 1177–90.Google Scholar
  3. Carrasco, F. H., Montes, G. S., Krisztan, R. M., Shigihara, K. M., Carneiro, J. &Junqueira, L. C. U. (1981) Comparative morphologic and histochemical studies on the collagen of vertebrate arteries.Blood Vessels,18, 296–302.Google Scholar
  4. Chung, E. &Miller, E. J. (1974) Collagen polymorphism: characterization of molecules with the chain composition [α1(III)]3 in human tissues.Science 183, 1200–1.Google Scholar
  5. Duance, V. C., Restall, D. J., Beard, H., Bourne, F. J. &Bailey, A. J. (1977) The location of three collagen types in skeletal muscle.FEBS Lett. 79, 248–52.Google Scholar
  6. Engel, U. R. (1971) Glycosaminoglycans in the aorta of six animal species.Atherosclerosis 13, 45–60.Google Scholar
  7. Gay, S., Balleison, L., Remberger, K., Fietzed, P. P., Adelman, B. C. &Kuhn, K. (1975) Immunohistochemical evidence for the presence of collagen type III in human arterial walls, arterial thrombi and in leukocytes, incubated with collagenin vitro.Klin. Wochenschr. 53, 899–902.Google Scholar
  8. Gay, S., Walter, P. &Kuhn, K. (1976) Characterization and distribution of collagen types in arterial heterografts originating from the calf carotid.Klin. Wochenschr. 54, 889–94.Google Scholar
  9. Junqueira, L. C. U., Cossermelli, W. &Brentani, R. (1978) Differential staining of collagen types, I, II and III by sirius red and polarization microscopy.Arch. Histol. Jpn. 41, 267–4.Google Scholar
  10. Laurent, G. J., Cockerill, P., McNaulty, R. J. &Hastings, J. R. B. (1981) A simplified method for quantification of the relative amounts of type I and type III collagen in small tissue samples.Anal. Biochem. 113, 301–12.Google Scholar
  11. Madri, J. A., Dreyer, B., Pitlick, F. A. &Furthmayr, H. (1980) The collagenous components of the subendothelium.Lab. Invest. 43, 303–15.Google Scholar
  12. McCullagh, K. G. &Balian, G. (1975) Collagen characterisation and cell transformation in human atherosclerosis.Nature 258, 73–75.Google Scholar
  13. McCullagh, K. G., Duance, V. C. &Bishop, K. A. (1980) The distribution of collagen types, I, III and V (AB) in normal and atherosclerotic human aorta.J. Pathol. 130, 45–55.Google Scholar
  14. Morton, L. F. &Barnes, M. J. (1982) Collagen polymorphism in the normal and diseased blood vessel wall.Atherosclerosis 42, 41–51.Google Scholar
  15. Murata, K. &Oshima, Y. (1975) Chondroitin sulfates in atherosclerotic human aorta.Atherosclerosis 21, 93–103.Google Scholar
  16. Rauterberg, J. (1974) Characterization of a genetic variant of collagen (type III) from calf aorta.Abstr. 4th Eur. Symp. Connect. Tissue Res., Padua, 106.Google Scholar
  17. Rucklidge, G. J., Riddoch, G. I. &Robins, S. P. (1986a) Immunocytochemical staining of rat tissue with antibodies to denatured type I collagen: a technique for localizing areas of collagen degradation.Collagen Relat. Res. 6, 41–49.Google Scholar
  18. Rucklidge, G. J., Milne, G., Riddoch, G. I. &Robins, S. P. (1986b) Evidence for renal tubular resorption of collagen fragments from immunostaining of rat kidney with antibodies specific for denatured type I collagen.Collagen Relat. Res. 6, 185–93.Google Scholar
  19. Stefanovich, V. &Akiyama, K. (1970) Comparative studies of aortic acid mucopolysaccharides in fifteen species.Comp. Biochem. Physiol. 34, 125–30.Google Scholar
  20. Toledo, O. M. S. &Mourao, P. A. S. (1980) Sultated glycosaminoglycans in normal aortic wall of different mammals.Artery 6, 341–53.Google Scholar
  21. Trestad, R. L. (1974) Human aorta collagens: Evidence for three distinct species.Biochem. Biophys. Res. Commun. 37, 717–25.Google Scholar
  22. Ward, H. A. &Fothergill, J. E. (1976)Fluorescent Protein Tracing, 4th edn (edited byNairn, R. L.), pp. 5–38. Edinburgh, London, New York: Churchill Livingstone.Google Scholar

Copyright information

© Chapman and Hall Ltd. 1989

Authors and Affiliations

  • Colin Farquharson
    • 1
  • Simon P. Robins
    • 1
  1. 1.Biochemistry DivisionRowett Research InstituteBucksburnScodand, UK

Personalised recommendations