Neurochemical Research

, Volume 19, Issue 10, pp 1237–1242

Transport of 3-hydroxybutyrate by cultured rat brain astrocytes

  • J. Tyson Tildon
  • Mary C. McKenna
  • Joseph H. StevensonJr.
Original Articles

DOI: 10.1007/BF01006812

Cite this article as:
Tildon, J.T., McKenna, M.C. & Stevenson, J.H. Neurochem Res (1994) 19: 1237. doi:10.1007/BF01006812

Abstract

It is well established that 3-hydroxybutyrate can serve as an energy source for the brain. Since substrate utilization may be regulated in part by transport across the cellular membrane, we investigated the uptake of 3-hydroxybutyrate by primary cultures of rat brain astrocytes. Measurement of the net uptake indicated a saturable system and a Lineweaver-Burke type plot was consistent with a single carrier-mediated mechanism with a Km of 6.03 mM and a Vmax of 32.7 nmol/30 seconds/mg protein. The rate of uptake at pH 6.2 was more than ten times the rate at pH 8.2, with the rate at pH 7.4 being intermediate between these values, suggesting the possibility of cotransport with H+ or exchange with OH (antiport). Mersalyl had only a slight effect on the transport of 3-hydroxybutyrate, suggesting that sulfhydryl groups are not involved in the transport of this monocarboxylic acid. Phenylpyruvate and α-ketoisocaproate also attenuated the transport, but lactate had only a marginal effect. These results suggest that the utilization of 3-hydroxybutyrate as an energy source by astrocytes is regulated in part by carrier-mediated transport and that the uptake system is different from the lactate transport system.

Key Words

3-Hydroxybutyrate transport astrocytes energy metabolism brain cells 

Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • J. Tyson Tildon
    • 1
    • 2
  • Mary C. McKenna
    • 1
  • Joseph H. StevensonJr.
    • 1
  1. 1.Department of PediatricsUniversity of Maryland School of MedicineBaltimore
  2. 2.Department of Biological ChemistryUniversity of Meryland School of MedicineBaltimore

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