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The Histochemical Journal

, Volume 8, Issue 1, pp 71–75 | Cite as

Resistance of aldehyde blockades to periodic acid

  • Olga B. Bayliss
  • C. W. M. Adams
Papers

Synopsis

Densitometric studies on rat liver, myelin and intestinal mucin showed that, among eight groups of reagents tested, only phenylhydrazines and aniline-acetic acid reliably blockaded aldehydes in such a way as to be reasonably resistant to re-oxidation by periodic acid. It is proposed, therefore, that only these agents among those tested should be used to block pre-existing aldehydes before performing the PAS reaction.

Keywords

Aldehyde Periodic Acid Phenylhydrazines Intestinal Mucin Densitometric Study 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Adams, C. W. M. &Bayliss, O. B. (1963). Histochemical observations on the localisation and origin of sphingoyelin, cerebroside and cholesterol in the normal and atherosclerotic human artery.J. Path. Bact. 85, 113–19.Google Scholar
  2. Culling, C. F. A. (1974).Handbook of Histopathological and Histochemical Techniques, 3rd Edn, p. 289 London: Butterworths.Google Scholar
  3. Danielli, J. F. (1949). A critical study of techniques for the cytochemical demonstration of aldehydes.Q. Jl. microsc. Sci. 90, 67–74.Google Scholar
  4. Peulgen, R. &Voit, K. (1924). Ueber einen Weitverbreiteten festen Aldehyd seine Entstehung auseiner Varstufe, sein mikrochemischer Nachweis und die Wege zu seiner praparativen Darstellung,Pflügers. Arch. ges. Physiol. 206, 389–410.Google Scholar
  5. Geyer, G. (1963). Histochemische Reduktion von aldehyden mit Natriumborohydrid.Acta histochem. 15, 1–5.Google Scholar
  6. Jobst, C. &Horváth, A. (1961). Contributory data on the reduction of oxo-groups in lipids.J. Histochem. Cytochem. 9, 711–12.Google Scholar
  7. Lillie, R. D. (1954).Histopathologic Technic and Practical Histochemistry, 2nd Edn, p. 159. New York: Blakiston.Google Scholar
  8. Lillie, R. D. &Glenner, G. G. (1957). Histochemical aldehyde blockade by aniline in glacial acetic acid.J. Histochem. Cytochem. 5, 167–9.Google Scholar
  9. Lillie, R. D. &Pizzolato, P. (1972). Histochemical use of borohydrides as aldehyde blocking reagents.Stain Technol. 47, 13–16.Google Scholar
  10. Oster, K. A. &Mulinos, M. G. (1944). Tissue aldehydes and their reaction with amines.J. Pharmacol. 80, 132–8.Google Scholar
  11. Pharse, A. G. E. (1968).Histochemistry, Theoretical and Applied, 3rd Edn, Vol. I, pp. 459, 706. London: Churchill.Google Scholar
  12. Staple, P. H. (1958). Effect of periodic acid on histochemical aldehyde blockades.Nature, Lond. 181, 288.Google Scholar

Copyright information

© Chapman and Hall Ltd 1976

Authors and Affiliations

  • Olga B. Bayliss
    • 1
  • C. W. M. Adams
    • 1
  1. 1.Department of PathologyGuy's Hospital Medical SchoolLondonUK

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