The Histochemical Journal

, Volume 16, Issue 3, pp 311–319

Complex carbohydrates in the secretory epithelium of the goat prostate

  • A. Tsukise
  • K. Yamada
Papers

Summary

The complex carbohydrates in the epithelium of the goat prostate were studied with a battery of light microscopical histochemical methods that included peroxidase-labelled lectin-diaminobenzidine (PO-LT-DAB) procedures. The secretory epithelial cells were found to be of three types: (a) mucus-producing cells, (b) protein-secreting cells, and (c) cells intermediate between (a) and (b). All these cell types contained variable amounts of neutral and acidic complex carbohydrates. The histophysiological significances of these substances in the glandular cells are discussed with special reference to the function of the accessory sex glands.

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References

  1. Aumüller, G. (1973) Über Sialomucin-produzierende Zellen der Prostata.Verh. Anat. Ges. 67, 233–43.Google Scholar
  2. Aumüller, G. (1979) Prostate gland. InHandbuch der Mikroskopischen Anatomie des Menschen, VII/6, Prostate gland and seminal vesicles (edited byAumüller, G.), pp. 53–182. Berlin, Heidelberg, New York: Springer-Verlag.Google Scholar
  3. Brandes, D. (1974) Fine structure and cytochemistry of male sex accessory organs. InMale Accessory Sex Organs (edited byBrandes, D.), pp. 17–114. New York, London: Academic Press.Google Scholar
  4. Cavazos, L. F. (1975) Fine structure and functional correlates of male accessory sex glands of rodents. InHandbook of Physiology (edited byHamilton, D. W. andGreep, R. O.), Vol. 5, pp. 358–81. Washington: American Physiological Society.Google Scholar
  5. Collard, J. G. &Temmink, J. H. M. (1974) Binding and cytochemical detection of cell-bound concanavalin A.Exp. Cell Res. 86, 81–6.Google Scholar
  6. Dellman, H.-D. &Wrobel, K.-H. (1976) Male reproductive system. InTextbook of Veterinary Histology (edited byDellman, H.-D. andBrown, E. M.) pp. 291–318. Philadelphia: Lea & Febiger.Google Scholar
  7. Franks, L. M., O'shea, J. D. &Thomson, A. E. R. C. (1964) Mucin in the prostate. A histochemical study in normal glands, latent, clinical and colloid cancers.Cancer 17, 983–91.Google Scholar
  8. Geuze, J. J. &Slot, J. W. (1978) Synthesis and secretion of glycoprotein in rat bulbo-urethral (Cowper's) glands.Am. J. Anat. 152, 391–418.Google Scholar
  9. Halbhuber, J. &Geyer, G. (1965) Histochemischer Nachweis von Sialomucin in den Glandulae bulbourethrales.Acta Histochem. 21, 148–49.Google Scholar
  10. Kiernan, J. A. (1975) Localization of α-d-glucosyl and α-d-mannosyl groups, of mucosubstances with concanavalin A and horseradish peroxidase.Histochemistry 44, 39–45.Google Scholar
  11. Leppi, T. J. (1968) Morphochemical analysis of mucous cells in the skin and slime glands of hagfishes.Histochemie 15, 68–78.Google Scholar
  12. Lev, R. &Spicer, S. S. (1964) Specific staining of sulfate groups with alcian blue at low pH.J. Histochem. Cytochem. 12, 309.Google Scholar
  13. Mann, T. (1964)The Biochemistry of Semen and of the Male Reproductive Tract. London: Methuen.Google Scholar
  14. Mann, T. (1975) Biochemistry of semen. InHandbook of Physiology (edited byHamilton, D. W. andGreep, R. O.), Vol. 5, pp. 461–71. Washington: American Physiological Society.Google Scholar
  15. Mcmanus, J. F. A. &Mowry, R. W. (1958) Effects of fixation on carbohydrate histochemistry.J. Histochem. Cytochem. 6, 309–16.Google Scholar
  16. Mowry, R. W. (1963) The special value of methods that colour with acidic and hydroxyl groups in the histochemical study of mucins. With revised directions for the colloidal iron stain, the use of Alcian Blue 8 GX and their combination with the periodic acid-Schiff reaction.Ann. N.Y. Acad. Sci. 106, 402–23.Google Scholar
  17. Nickel, R., Schummer, A. &Seiferle, E. (1967)Lehrbuch, der Anatomie der Haustiere. Bd. II, pp. 307–54. Berlin & Hamburg: Paul Parey.Google Scholar
  18. Nielsen, E. H. (1976) The bulbourethral gland of the rat. Fine structure and histochemistry.Anat. Anz. 139, 254–63.Google Scholar
  19. Pearse, A. G. E. (1968)Histochemistry, Theoretical, and Applied. Vol. 1. London: J. & A. Churchill.Google Scholar
  20. Spicer, S. S. (1960) A correlative study of the histochemical properties of rodent acid mucopolysaccharides.J. Histochem. Cytochem. 8, 18–35.Google Scholar
  21. Spicer, S. S. (1965) Diamine methods for differentiating mucosubstances histochemically.J. Histochem. Cytochem. 13, 211–43.Google Scholar
  22. Spicer, S. S., Horn, R. G. &Leppi, T. J. (1967) Histochemistry of connective tissue mucopolysaccharides. InThe Connective Tissue (edited byWagner, B. M. andSmith, D. E.) pp. 251–303. Baltimore: Williams & Wilkins.Google Scholar
  23. Stoward, P. J., Spicer, S. S. &Miller, R. L. (1980) Histochemical reactivity for peanut lectin-horseradish peroxidase conjugate.J. Histochem. Cytochem. 28, 939–90.Google Scholar
  24. Tsukise, A. &Yamada, K. (1981a) The histochemistry of complex carbohydrates in the epithelium lining the ventral prostate of the rat.Histochemistry 72, 215–27.Google Scholar
  25. Tsukise, A. &Yamada, K. (1981b) The histochemistry of complex carbohydrates in the scrotum of the boar.Histochemistry 72, 511–21.Google Scholar
  26. Tsukise, A. & Yamada, K. (1983) The histochemistry of complex carbohydrates in the epithelium lining the prostate, seminal vesicle and bulbourethral glands of the boar.Proceedings of the 87th Meeting Japanese Association of Anatomists 73.Google Scholar
  27. Tsukise, A., Sugawa, Y. &Yamada, K. (1979) Histochemistry of carbohydrates in the epithelium lining the bulbourethral gland of the rat.Acta anat 105, 529–38.Google Scholar
  28. Wrobel, K.-H. (1970) Feinstruktur und histochemie der Glandula bulbourethralis der Ziege.Z. Zellforsch. 108, 582–96.Google Scholar
  29. Wrobel, K.-H. (1972) Histochemische Untersuchungen am Prostata-parenchym der Ziege.Zbl. Vet. Med. C1, 64–72.Google Scholar
  30. Wrobel, M. (1972) Histochemische und elektronenmikroskopische Untersuchungen an der Prostata von Hund und Katze.Zbl. Vet. Med. C1, 93–116.Google Scholar
  31. Yamada, K. (1972) Histochemical and cytochemical methods for the study of mucosaccharides.Protein, Nucleic Acid and Enzyme 17, 775–90.Google Scholar
  32. Yamada, K. (1973) The effect of digestion withStreptomyces hyaluronidase upon certain histochemical reactions of hyaluronic acid-containing tissue.J. Histochem. Cytochem. 21, 794–803.Google Scholar
  33. Yamada, K. &Hoshino, M. (1972) Morphochemical, analysis of epithelial mucosubstances in the gall bladder of the fowl (Gallus domesticus).Histochemie 29, 120–28.Google Scholar
  34. Yamada, K. &Shimizu, S. (1977) The histochemistry of galactose residues of complex carbohydrates as studied by peroxidase-labelledRicinus communis agglutinin.Histochemistry 53, 143–56.Google Scholar
  35. Yamada, K. &Shimizu, S. (1979) The use of peroxidase-labelledLimulus polyphemus agglutinin for the histochemistry of sialic acid-containing glycoproteins in light microscopy.Histochem. J. 11, 457–71.Google Scholar

Copyright information

© Chapman and Hall Ltd 1984

Authors and Affiliations

  • A. Tsukise
    • 1
  • K. Yamada
    • 2
  1. 1.Department of Veterinary Anatomy, College of Agriculture and Veterinary MedicineNihon UniversityEujisawa, KanagawaJapan
  2. 2.Department of AnatomyNagoya City University, Medical SchoolNagoyaJapan

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