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The Histochemical Journal

, Volume 2, Issue 1, pp 87–89 | Cite as

The PASDORO method for simultaneously demonstrating DNA and lipids in the brain

  • O. B. Bayliss
  • C. W. M. Adams
  • J. F. Hallpike
Short Communication

Conclusions and summary

The PASDORO method has the advantage of allowing DNA and globular lipid to be demonstrated in frozen sections of formalin-fixed tissue. Its disadvantage is that, in most tissues, basement membrane staining interferes with the selective demonstration of DNA. However, in the central nervous system it is advantageous to be, able to trace capillary basement membrane. In this way cells related to the capillary endothelium can be easily identified. The addition of staining with Oil Red O to the basic PASD technique permits lipid-laden microglia to be readily identified around necrotic and demyelinating lesions.

Keywords

Lipid Nervous System Central Nervous System Basement Membrane Freeze Section 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Adams, C. W. M., Bayliss, O. B. &Weller, R. O. (1965). The preferential demonstration of deoxyribonucleic acid by a periodic acid-silver diamine method for light and electron microscopy.J. Histochem Cytochem. 13, 694–7.Google Scholar
  2. Lillie, R. D. (1954).Hispatholigic Technic and Practical Histochemistry, p. 335. New York: Blakiston.Google Scholar

Copyright information

© Chapman and Hall Ltd 1970

Authors and Affiliations

  • O. B. Bayliss
    • 1
  • C. W. M. Adams
    • 1
  • J. F. Hallpike
    • 1
  1. 1.Department of PathologyGuy's Hospital Medical SchoolLondon

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