The PASDORO method for simultaneously demonstrating DNA and lipids in the brain
- 9 Downloads
Conclusions and summary
The PASDORO method has the advantage of allowing DNA and globular lipid to be demonstrated in frozen sections of formalin-fixed tissue. Its disadvantage is that, in most tissues, basement membrane staining interferes with the selective demonstration of DNA. However, in the central nervous system it is advantageous to be, able to trace capillary basement membrane. In this way cells related to the capillary endothelium can be easily identified. The addition of staining with Oil Red O to the basic PASD technique permits lipid-laden microglia to be readily identified around necrotic and demyelinating lesions.
KeywordsLipid Nervous System Central Nervous System Basement Membrane Freeze Section
Unable to display preview. Download preview PDF.
- Adams, C. W. M., Bayliss, O. B. &Weller, R. O. (1965). The preferential demonstration of deoxyribonucleic acid by a periodic acid-silver diamine method for light and electron microscopy.J. Histochem Cytochem. 13, 694–7.Google Scholar
- Lillie, R. D. (1954).Hispatholigic Technic and Practical Histochemistry, p. 335. New York: Blakiston.Google Scholar