The Histochemical Journal

, Volume 10, Issue 5, pp 573–584 | Cite as

Concanavalin A-peroxidase-diaminobenzidine-periodic acid-m-aminophenol-Fast Black salt K: a method for the dual staining of neutral complex carbohydrates

  • Kazuyori Yamada
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Synopsis

A method has been developed for the dual staining of neutral complex carbohydrates in light microscopy. It combines a concanavalin A-peroxidase-diaminobenzidine (Con A-PO-DAB) method with a period acid-m-aminophenol-Fast Black salt K (PA-AP-FBK) sequence. With the combined method it is possible to stain α-D-glycosyl and α-D-mannosyl residues brown and 1,2-glycol groups of neutral complex carbohydrates blackish purple. The validity of the method has been confirmed with appropriate histochemical controls and enzyme digestions on test tissues.

Keywords

Carbohydrate Light Microscopy Enzyme Digestion Concanavalin Combine Method 
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Copyright information

© Chapman and Hall Ltd. 1978

Authors and Affiliations

  • Kazuyori Yamada
    • 1
  1. 1.Laboratory of Histology, Department of AnatomyNagoya University School of MedicineNagoyaJapan

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