Cyclic 3′,5′-adenosine monophosphate level and adenylate cyclase activity in human blood platelets during storage in ACD solution
Summary
The level of cyclic 3′,5′-adenosine monophosphate (cAMP) in human blood platelets and the activity of platelet adenylate cyclase in response to prostaglandin E1 stimulation do not change during two days storage at room temperature in ACD solution. However, the level of cyclic AMP is lower in platelets stored in ACD solution than in platelets from blood anticoagulated by ethylenediamine tetra-acetic acid.
Key words
Platelet storage cAMP prostaglandin E1 ACD EDTAZusammenfassung
Die Konzentration von zyklischem AMP und die Aktivität der Adenylat-Zyklase nach Stimulation mit Prostaglandin E1 in menschlichen Thrombozyten ändert sich nicht, wenn diese zwei Tage bei Zimmertemperatur in ACD-Lösung gelagert werden. Die Konzentration von zyklischem AMP in derart gelagerten Blutplättchen ist niedriger als in Thrombozyten, die aus EDTA ungerinnbar gemachtem Blut gewonnen werden.
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References
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