Neurochemical Research

, Volume 20, Issue 9, pp 1107–1113

Specificity of zinc binding to myelin basic protein

  • P. Riccio
  • S. Giovannelli
  • A. Bobba
  • E. Romito
  • A. Fasano
  • T. Bleve-Zacheo
  • R. Favilla
  • E. Quagliariello
  • P. Cavatorta
Article

DOI: 10.1007/BF00995566

Cite this article as:
Riccio, P., Giovannelli, S., Bobba, A. et al. Neurochem Res (1995) 20: 1107. doi:10.1007/BF00995566

Abstract

Z2+ appears to stabilize the myelin sheath but the mechanism of this effect is unknown. In a previous report we have shown that zinc binds to CNS myelin basic protein (MBP) in the presence of phosphate and this results in MBP aggregation. For this paper we used a solid phase zinc blotting assay to identify which myelin proteins bind zinc. MBP and a 58 kDa band were found to be the major targets of65Zn binding. Moreover, using fluorescence, light scattering and electron microscopy we investigated the binding of zinc and other cations to purified MBP in solution. Among the cations tested for their ability to interfere with the binding of zinc, the most effective were cadmium, mercury and copper, but only cadmium and mercury increased the scattering intensity, whereas MBP aggregation was not inhibited by copper ions. Thus, the effect of zinc on the formation of MBP clusters seems to be specific.

Key Words

Myelin myelin basic protein zinc myelin structure cations phosphorylation 

Copyright information

© Plenum Publishing Corporation 1995

Authors and Affiliations

  • P. Riccio
    • 1
    • 3
  • S. Giovannelli
    • 2
  • A. Bobba
    • 3
  • E. Romito
    • 4
  • A. Fasano
    • 4
  • T. Bleve-Zacheo
    • 5
  • R. Favilla
    • 2
  • E. Quagliariello
    • 4
  • P. Cavatorta
    • 2
  1. 1.Dept. of Biology, Defence and A.F. TechnologyUniversity of BasilicataPotenzaItaly
  2. 2.Dept. of Physics, Section of BiophysicsUniversity of ParmaParmaItaly
  3. 3.Center for the Study of Mitochondria and Energetic Metabolism, National Council for Research (CNR)University of BariBariItaly
  4. 4.Dept. of Biochemistry and Molecular BiologyBariItaly
  5. 5.Ints. of NematologyCNRBariItaly

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