Advertisement

Zoomorphologie

, Volume 93, Issue 3, pp 193–207 | Cite as

Electron microscopical and histochemical studies of differentiation and function of the cephalopod gill (Sepia officinalis L.)

  • Rudolf Schipp
  • Stephan Mollenhauer
  • Sigurd von Boletzky
Article

Summary

Phase contrast and electron microscopical investigations on the gill ofSepia officinalis L., from the stage shortly before hatching to the adult stage, demonstrate (1) peripheral respiratory epithelial areas and (2) highly folded epithelia rich in mitochondria in the concave recesses of the gill lamellae. Enzyme-histochemical and cytochemical findings, and in particular the GOT proof, suggest by analogy with the chloride cells of the teleosts that the cells of this second type of epithelium are capable of active transport, and that they probably secrete ammonia. Apparently the gill of cephalopods, too, serves both respiratory and excretory functions.

Keywords

Chloride Ammonia Developmental Biology Active Transport Phase Contrast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Boletzky, S. von: Effets de la sous-nutrition prolongée sur le développement de la coquille deSepia officinalis L. (Mollusca, Cephalopoda). Bull. Soc. Zool. France99, 667–673 (1974)Google Scholar
  2. Burck, H.C.: Histologische Technik. Stuttgart: Thieme-Verlag, 1973Google Scholar
  3. Burstone, M.S.: New histochemical technique for the demonstration of tissue oxidase (cytochrome oxidase). J. Histochem. Cytochem.7, 112–122 (1959)Google Scholar
  4. Cuvier, G.: Le règne animal. Les céphalopodes: Paris 1817Google Scholar
  5. Deimling, O.H. von: Die Darstellung phosphatfreisetzender Enzyme mittels Schwermetall-Simultanmethode. Histochemie4, 48–55 (1964)Google Scholar
  6. Donaubauer, H.H., Schipp, R.: Localization of enzyme activities in the pancreatic appendages ofSepia officinalis L. (Cephalopoda). Histochemistry59, 29–44 (1978)Google Scholar
  7. Glenner, G.G., Burtner, H.J., Brown, G.W.: The histochemical demonstration of monoaminoxidase activity by tetrazolium salts. J. Histochem. Cytochem.5, 591–600 (1957)Google Scholar
  8. Goebel, A., Puchtler, H.: Zur Darstellung der Carboanhydrase im histologischen Schnitt. Naturwissenschaften41, 531–532 (1954b)Google Scholar
  9. Gomori, G.: An improved histochemical technique for acid phosphatase. Stain Technol.25, 81–85 (1950)Google Scholar
  10. Gomori, G.: The complex nature of alkaline phosphatase. Biochem. biophys. Acta (Amst.)8, 162–172 (1952)Google Scholar
  11. Joubin, L.: Structure et développement de la branchie de quelques Céphalopodes des côtes de France. Arch. Zool. exp. gén. (2e série),3, 75–150 (1885)Google Scholar
  12. Karnovsky, M.J., Himmelhoch, S.R.: Histochemical localization of glutaminase I activity in kidney. Am. J. Physiol.201, 786–790 (1961)Google Scholar
  13. Lee, Sin Hang: The possible role of the vesicles in renal ammonia excretion. J. of Cell Biol.45, 644–649 (1970)Google Scholar
  14. Lolova, I., Dikov, A.: Histochemical evidence of aminotransferase. IV. Histochemical and electrophoretical investigation of aminotransferase in rat organs. Acta Histochem.53, 12–27 (1975)Google Scholar
  15. Magnun, C.P., Dykens, J.A., Henry, R.P., Polites, G.E.: The excretion of NH4+ and its ouabain sensitivity in aquatic annelids and molluscs (1). J. Exp. Zool.203, 151–159 (1978)Google Scholar
  16. Newstead, J.D.: Fine structure of the respiratory lamellae of teleostean gills. Z. Zellforschung79, 396–428 (1967)Google Scholar
  17. Pearse, A.G.E.: Histochemistry. Theoretical and applied. 2nd and 3rd Ed. London: Churchill 1961/1968Google Scholar
  18. Philpott, C.W. and Copeland, D.E.: Fine structure of chloride cells from three species ofFundulus. J. Cell Biol.18, 389 (1963)Google Scholar
  19. Potts, W.T.W.: Ammonia excretion inOctopus dofleini. Comp. Biochem. Physiol.16, 479–489 (1965)Google Scholar
  20. Robertson, J.D.: Further studies on ionic regulation in marine invertebrates. J. Exp. Biol.30, 277–296 (1953)Google Scholar
  21. Sasse, D.: Glykogen in der Ontogenese des Verdauungstraktes — Chemomorphologische und stoffwechselhistochemische Analyse. Ergebn. Anat. Entwick.-Gesch.40/2, 1–68 (1968)Google Scholar
  22. Schäfer, P.: Über die Atmungsorgane der tetra- und dibranchiaten Cephalopoden. Inaug. Diss. Leipzig (1904)Google Scholar
  23. Schäfer, A., Schipp, R.: Vergleichende elektronenmikroskopische Untersuchungen an den Kiemen verschiedener Teleostier zur osmoregulatorischen Funktion der Chloridzellen. Verh. der Anat. Ges. Leipzig 1968, Anat. Anz.125, 277–289 (1969)Google Scholar
  24. Schipp, R., Schäfer, A.: Die Besonderheiten der Feinstruktur von Kiemenherz und Kiemengefäßen von Cephalopoden. Verh. der Deutsch. Zool. Ges. in Innsbruck (1968)Google Scholar
  25. Schipp, R., Höhn, P., Schäfer, A.: Elektronenmikroskopische und histochemische Untersuchungen zur Funktion des Kiemenherzanhangs (Pericardialdrüse) von Sepia officinalis. Z. Zellforschung117, 252–274 (1971)Google Scholar
  26. Schipp, R., Boletzky, S. von: Morphology and function of the excretory organs in dibranchiate Cephalopods. Fortschritte der Zoologie23, 2/3 (1975)Google Scholar
  27. Schipp, R., Boletzky, S. von, Doell, G.: Ultrastructural and cytochemical investigations on the renal appendages and their concrements in dibranchiate cephalopods (Mollusca, Cephalopoda). Z. Morph. Tiere81, 279–304 (1975)Google Scholar
  28. Schoffeniels, E.: Isolation of a sodium-dependent ATPase from the gills ofOctopus vulgaris L. Life Sciences9, 437–440 (1962)Google Scholar
  29. Seligman, A.M., Kwang-Chung Tson, Rutenberg, S.H., Cohen, R.B.: Histochemical demonstration ofβ-D-glucuronidase with a synthetic substrate. J. Histochem. Cytochem.2, 209–229 (1954)Google Scholar
  30. Tilesius von Tillenau, W.G.: De respirationeSepia officinalis. Diss. Univ. Lipsiae, 88 pp (1861)Google Scholar
  31. Tompsett, D.H.: Sepia. Univ. Press of Liverpool 1939Google Scholar
  32. Towle, D.W., Gilman, M.E., Hempel, J.D.: Rapid modulation of gill Na++K+-dependent ATPase activity during acclimation of the killifishFundulus heteroclitus to salinity change. J. Exp. Zool.202, 2, 179–213 (1977)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Rudolf Schipp
    • 1
    • 2
    • 3
  • Stephan Mollenhauer
    • 1
    • 2
    • 3
  • Sigurd von Boletzky
    • 1
    • 2
    • 3
  1. 1.I. Zoologisches Institut der Justus-Liebig-UniversitätGiessenGermany
  2. 2.Laboratoire AragoC.N.R.S.Banyuls-sur-Mer
  3. 3.Station de Biologie MarineArcachonFrance

Personalised recommendations