Journal of Chemical Ecology

, Volume 18, Issue 2, pp 127–136 | Cite as

A nematicidal toxin fromPleurotus ostreatus NRRL 3526

  • O. C. H. Kwok
  • R. Plattner
  • D. Weisleder
  • D. T. Wicklow


A nematicidal toxin was purified fromPleurotus ostreatus NRRL 3526 grown on moistened, autoclaved wheat straw for 30 days at room temperature (21–33°C). The active compound, at a concentration of 300 ppm, immobilized 95% of test nematodes (Panagrellus redivivus) within 1 hr. Immobilized nematodes did not recover, even after being rinsed with deionized water. The toxin was identified astrans-2-decenedioic acid.

Key words

trans-2-Decenedioic acid Pleurotus ostreatus fungus nematotoxin Panagrellus redivivus fatty acids ostreatin dicarboxylic acids natural nematicides nematoda Penagrellus redivivus 


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  1. Balan, J., andGerber, N.N. 1972. Attraction and killing of the nematodePanagrellus redivivus by the predaceous fungusArthrobotrys dactyloides.Nematologia 18:163–173.Google Scholar
  2. Balan, J., Krizkova, L., Nemec, P., andVollek, V. 1974. Production of nematode-attracting and nematicidal substances by predacious fungi.Folia Microbiol. 19:512–519.Google Scholar
  3. Barron, G.L. 1977. The Nematode-Destroying Fungi. Canadian Biological Publications, Guelph, Ontario.Google Scholar
  4. Barron, G.L., andThorn, R.G. 1987. Destraction of nematodes by species ofPleurotus.Can. J. Bot. 65:774–778.Google Scholar
  5. Brenner, S. 1974. The genetics ofCaenorhabditis elegans.Genetics 77:71–94.Google Scholar
  6. Cram, D.J., andTishler, M. 1948. Mold metabolites. I. Isolation of several compounds from clinical penicillin.J. Am. Chem. Soc. 70:4238–4239.Google Scholar
  7. Fogleman, J.C., andKircher, H.W. 1986. Differential effects of fatty acid chain length on the viability of two species of catophilicDrosophila.Comp. Biochem. Physiol. 83A:761–764.Google Scholar
  8. Freese, E. 1978. Mechanism of growth inhibition by lipophilic acids, pp. 123–131,in J.J. Kabara (ed.). The Pharmacological Effects of Lipids. The American Oil Chemists' Society, Champaign, Illinois.Google Scholar
  9. Giuma, A.Y., andCooke, R.C. 1971. Nematotoxin production byNematoctonus haptocladus andN. concurrens.Trans. Br. Mycol. Soc. 56:89–94.Google Scholar
  10. Giuma, A.Y., Hackett, A.M., andCooke, R.C. 1973. Thermostable nematotoxins produced by germinating conidia of some endozoic fungi.Trans. Br. Mycol. Soc. 60:49–56.Google Scholar
  11. Hunkova, Z., andFencl, Z. 1977. Toxic effects of fatty acids on yeast cells: Dependence of inhibitory effects of fatty acid concentration.Biotechnol. Bioeng. 19:1623–1641.Google Scholar
  12. Kennedy, N., andTampion, J. 1978. A nematotoxin fromNematoctonus robustus.Trans. Br. Mycol. Soc. 70:140–141.Google Scholar
  13. Krizkova, L., Balan, J., Nemec, P., andKolozvary, A. 1976. Predaceous fungiDactylaria pyriformis andD. thaumasia: Production of attractants and nematicides.Folia Microbiol. 21:493–494.Google Scholar
  14. Munakata, K. 1983. Nematicidal natural products, pp. 299–311, in D.L. Whitehead and W.S. Bowers (eds.). Natural Products for Innovative Pest Management. Pergamon Press, New York.Google Scholar
  15. Olthof, T.H.A., andEstey, R.H. 1963. A nematotoxin produced by the nematophagous fungusArthrobotrys oligospora Fresenius.Nature 197:514–515.Google Scholar
  16. Puritch, G.S. 1978. Biocidal effects of fatty acid salts on various forest insect pest, pp. 105–112, in J.J. Kabara (ed.). The Pharmacological Effects of Lipids. The American Oil Chemists' Society, Champaign, Illinois.Google Scholar
  17. Teh, J.S. 1974. Toxicity of short-chain fatty acids and alcohols towardsCladosporium resinae.Appl. Microbiol. 28:840–844.Google Scholar
  18. Thorn, R.G., andBarron, G.L. 1984. Carnivorous mushrooms.Science 224:76–78.Google Scholar
  19. Wurzenberger, M., andGrosch, W. 1986. Enzymic oxidation of linolenic acid to 1,Z-5-octadien-3-ol,Z-2,Z-5-octadien-1-ol and 10-oxo-E-8-decenoic acid by a protein fraction from mushrooms (Psalliota bispora).Lipids 21:261–266.Google Scholar

Copyright information

© Plenum Publishing Corporation 1992

Authors and Affiliations

  • O. C. H. Kwok
    • 1
  • R. Plattner
    • 1
  • D. Weisleder
    • 1
  • D. T. Wicklow
    • 1
  1. 1.National Center for Agricultural Utilization ResearchUSDA-Agricultural Research Service-Midwest AreaPeoria

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