Plant Systematics and Evolution

, Volume 209, Issue 1–2, pp 85–91 | Cite as

Flow cytometric measurement of nuclear DNA content inCapsicum (Solanaceae)

  • P. Belletti
  • C. Marzachì
  • S. Lanteri


Nuclei were isolated from leaf tissue of differentCapsicum species and the relative fluorescence intensity was measured by flow cytometry after propidium iodide staining.Pisum sativum nuclei with known nuclear genome size (9.07 pg) were used as internal standard to determine nuclear DNA content of the samples in absolute units. The 2C DNA contents ranged between 7.65 pg inC. annuum and 9.72 pg inC. pubescens, and the general mean of the genus was 8.42 pg. These values correspond, respectively, to 1C genome size of 3.691 (C. annuum), 4.690 (C. pubescens) and 4.063 (general mean) Mbp. In general, white-flowered species proved to have less DNA, with the exception ofC. praetermissum, which displayed a 2C DNA content of 9.23 pg. It was possible to divide the studied species into three main groups according to their DNA content, and demonstrate differences in DNA content within two of the three species complexes established on the basis of morphological traits.

Key words

Solanaceae Capsicum DNA content genome size flow cytometry 


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  1. Arumuganathan, K., Earle, E. D., 1991: Nuclear DNA content of some important plant species. — Pl. Molec. Biol. Reporter9: 208–218.Google Scholar
  2. Bagwell, C. B., Baker, B., Whetstone, S., Munson, M., Hitchcox, S., Aultu, K. A., Lovett, E. J., 1989: A simple and rapid method for determining the linearity of a flow cytometer amplification system. — Cytometry10: 689–694.Google Scholar
  3. Bennett, M. D., Smith, J. D., 1976: Nuclear DNA amounts in angiosperms. — Philos. Trans., Ser. B274: 227–274.Google Scholar
  4. Doležel, J., Sgorbati, S., Lucretti, S., 1992: Comparison of three DNA fluorochromes for flow cytometric estimation of nuclear DNA content in plants. — Physiol. Pl. (Copenhagen)85: 625–631.Google Scholar
  5. Galbraith, D. W., 1989: Analysis of higher plants by flow cytometry and cell sorting. — Int. Rev. Cytol.116: 165–228.Google Scholar
  6. , 1983: Rapid flow cytometric analysis of the cell cycle in intact plant tissues. — Science220: 1049–1051.Google Scholar
  7. International Board for Plant Genetic Resources, 1983: Genetic resources ofCapsicum — Rome: IBPGR.Google Scholar
  8. Lanteri, S., Pickersgill, B., 1993: Chromosomal structural changes inCapsicum annuum L. andC. chinense Jacq. — Euphytica67: 155–160.Google Scholar
  9. Moscone, E. A., Lambrou, M., Hunziker, A. T., Ehrendorfer, F., 1993: Giemsa C-banded karyotypes inCapsicum (Solanaceae). — Pl. Syst. Evol.186: 213–229.Google Scholar
  10. , 1995: Analysis of active nucleolus organizing regions inCapsicum (Solanaceae) by silver staining. — Amer. J. Bot.82: 276–287.Google Scholar
  11. Naci Onus, A., Pickersgill, B., 1995: Unilateral incompatibility inCapsicum. — In: 1st international symposium onSolanaceae for fresh market, Malaga, Spain, 28–31 March 1995, Abstracts, p. 14.Google Scholar
  12. Pickersgill, B., 1971: Relationship between weedy and cultivated forms in some species of chili peppers (genusCapsicum). — Evolution25: 683–691.Google Scholar
  13. , 1991: Cytogenetics and evolution ofCapsicum L. — InTsuchiya, T., Gupta, P. K., (Eds): Chromosome engineering in plants: genetics, breeding, evolution, part B, pp. 139–160. — Amsterdam: Elsevier.Google Scholar
  14. - 1992: Barriers to interspecific gene exchange inCapsicum. — InBelletti, P., Quagliotti, L., (Eds): Proceedings of the VIIIth meeting on genetics and breeding onCapsicum and eggplant. — Capsicum Newsletter, special issue, pp. 57–60.Google Scholar
  15. , 1979: Numerical taxonomic studied on variation and domestication in some species ofCapsicum. — InHawkes, J. G., Lester, R. N., Skelding, A. D., (Eds): The biology and taxonomy of theSolanaceae, pp. 679–700. — London: Academic Press.Google Scholar
  16. Pochard, E., Palloix, A., Daubeze, A. M., 1986: The use of androgenetic autodiploid lines for the analysis of complex resistance systems in pepper. — InDiputacion General de Aragon, (Ed): VIth meeting on genetics and breeding onCapsicum and eggplant, pp. 105–110. — Zaragoza.Google Scholar
  17. Saxena, P. K., King, J., 1989: Isolation of nuclei and their transplantation into plant protoplast. — InBajaj, Y. P. S., (Ed.): Biotechnology in agriculture and forestry. Plant protoplast and genetic engineering, pp. 328–342. — Berlin, Heidelberg, New York: Springer.Google Scholar
  18. Shopova, M., 1966: Studies in the genusCapsicum. I. Species differentiation. — Chromosoma19: 340–348.Google Scholar
  19. SPSS Inc., 1988: SPSS/PC+ Advanced Statistics V 20. — Chicago: SPSS Inc.Google Scholar

Copyright information

© Springer-Verlag 1998

Authors and Affiliations

  • P. Belletti
    • 1
  • C. Marzachì
    • 1
  • S. Lanteri
    • 1
  1. 1.Dipartimento di Valorizzazione e Protezione delle Risorse Agroforestali, Plant Breeding and Seed ProductionUniversity of TurinTorinoItaly

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