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Plant Systematics and Evolution

, Volume 209, Issue 1–2, pp 85–91 | Cite as

Flow cytometric measurement of nuclear DNA content inCapsicum (Solanaceae)

  • P. Belletti
  • C. Marzachì
  • S. Lanteri
Article

Abstract

Nuclei were isolated from leaf tissue of differentCapsicum species and the relative fluorescence intensity was measured by flow cytometry after propidium iodide staining.Pisum sativum nuclei with known nuclear genome size (9.07 pg) were used as internal standard to determine nuclear DNA content of the samples in absolute units. The 2C DNA contents ranged between 7.65 pg inC. annuum and 9.72 pg inC. pubescens, and the general mean of the genus was 8.42 pg. These values correspond, respectively, to 1C genome size of 3.691 (C. annuum), 4.690 (C. pubescens) and 4.063 (general mean) Mbp. In general, white-flowered species proved to have less DNA, with the exception ofC. praetermissum, which displayed a 2C DNA content of 9.23 pg. It was possible to divide the studied species into three main groups according to their DNA content, and demonstrate differences in DNA content within two of the three species complexes established on the basis of morphological traits.

Key words

Solanaceae Capsicum DNA content genome size flow cytometry 

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Copyright information

© Springer-Verlag 1998

Authors and Affiliations

  • P. Belletti
    • 1
  • C. Marzachì
    • 1
  • S. Lanteri
    • 1
  1. 1.Dipartimento di Valorizzazione e Protezione delle Risorse Agroforestali, Plant Breeding and Seed ProductionUniversity of TurinTorinoItaly

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