Advertisement

Plant Systematics and Evolution

, Volume 201, Issue 1–4, pp 75–82 | Cite as

The karyotype ofFestucopsis serpentini (Poaceae Triticeae) from Albania studied by banding techniques and in situ hybridization

  • Ib Linde-Laursen
  • Ole Seberg
  • Signe Frederiksen
  • Claus Baden
Article

Abstract

The karyotypes of two populations ofFestucopsis serpentini (2n = 2x = 14) endemic to Albania were investigated in detail by Giemsa C- and N-banding, AgNO3 staining, and in situ hybridization with an rDNA probe. The complements consisted of 14 large chromosomes, 10 metacentric and 4 SAT-chromosomes, a metacentric and a submetacentric pair. SAT-chromosomes from one population carried exclusively minute satellites, whereas SAT-chromosomes from another population also carried larger polymorphic satellites, suggesting a geographical differentiation. The existence of four chromosomes with nucleolus forming activity was established through AgNO3 staining; however, the rDNA probe additionally hybridized to intercalary positions in the short arms of two metacentric chromosomes revealing two inactive rDNA sites. C-banding patterns comprised from zero and up to four very small to larger, generally telomeric bands per chromosome giving low levels of constitutive heterochromatin. Similarities in chromosome morphology and C-banding patterns identified the homologous relationships of all chromosomes in one population, but of three pairs only in the other. Reliable identification of homologous chromosomes between plants was only possible for the SAT-chromosomes. A comparison between the C-banded karyotypes ofF. serpentini andPeridictyon sanctum supports their position in two genera.

Key words

Poaceae Triticeae Festucopsis Peridictyon Giemsa C-banding N-banding silver staining in situ hybridization FISH karyotype chromosome polymorphism nucleoli rDNA 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Baden, C., Linde-Laursen, I., Dewey, D. R., 1990: A new Chinese species ofPsathyrostachys (Poaceae) with notes on its karyotype. — Nordic J. Bot.9: 449–460.Google Scholar
  2. Baltisberger, M., Leuchtmann, A., 1991: Investigations on someGramineae from Albania and Greece (Chromosome numbers and endophyte infection). — Ber. Geobot. Inst. ETH, Stiftung Rübel, Zürich,57: 182–192.Google Scholar
  3. Endo, T. R., Gill, B. S., 1984: The heterochromatin distribution and genome evolution in diploid species ofElymus andAgropyron. — Canad. J. Genet. Cytol.26: 669–678.Google Scholar
  4. Gerlach, W. L., Bedbrook, J. R., 1979: Cloning and characterization of ribosomal RNA genes from wheat and barley. — Nucleic Acids Res.7: 1869–1885.PubMedGoogle Scholar
  5. Gill, B. S., 1987: Chromosome banding methods, standard chromosome band nomenclature, and applications in cytogenetic analysis. — InHeyne, E. G., (Ed.): Wheat and wheat improvement. 2nd edn, pp. 243–254. — Agronomy Monograph13. — Madison: ASA-CSSA-SSSA.Google Scholar
  6. —, 1991: Standard karyotype and nomenclature system for description of chromosome bands and structural aberrations in wheat (Triticum aestivum). — Genome34: 830–839.Google Scholar
  7. Hsiao, C., Wang, R. R.-C., Dewey, D. R., 1986: Karyotype analysis and genome relationships of 22 diploid species in the tribeTriticeae. — Canad. J. Genet. Cytol.28: 109–120.Google Scholar
  8. —, 1995: Phylogenetic relationships of the monogenomic species of the wheat tribe,Triticeae (Poaceae), inferred from nuclear rDNA (internal transcribed spacer) sequences. — Genome38: 211–223.PubMedGoogle Scholar
  9. Leitch, I. J., Heslop-Harrison, J. S., 1992: Physical mapping of the 18S-5.8S-26S rRNA genes in barley by in situ hybridization. — Genome35: 1013–1018.Google Scholar
  10. Levan, A., Fredga, K., Sandberg, A. A., 1965: Nomenclature for centromeric position on chromosomes. — Hereditas52: 201–220.Google Scholar
  11. Linde-Laursen, I., 1981: Giemsa banding patterns of the chromosomes of cultivated and wild barleys. — InAsher, M. J. C., Ellis, R. P., Hayter, A. M., Whitehouse, R. N. H., (Eds): Barley genetics,IV, pp. 786–795. — Edinburgh: Edinburgh University Press.Google Scholar
  12. —, 1984: Nucleolus organizer polymorphism in barley,Hordeum vulgare L. — Hereditas100: 33–43.Google Scholar
  13. —, 1994a: Comparison of the Giemsa C-banded karyotypes of the three subspecies ofPsathyrostachys fragilis, subspp.villosus (2x),secaliformis (2x, 4x), andfragilis (2x) (Poaceae), with notes on chromosome pairing. — Pl. Syst. Evol.191: 183–198.Google Scholar
  14. —, —, 1994b: Giemsa C-banded karyotypes of two cytotypes (2x, 4x) ofPsathyrostachys lanuginosa (Poaceae; Triticeae). — Hereditas120: 113–120.Google Scholar
  15. —, 1984: Identification of the somatic chromosomes ofPsathyrostachys fragilis (Poaceae). — Canad. J. Genet. Cytol.26: 430–435.Google Scholar
  16. —, —, 1986: Comparison of the karyotypes ofPsathyrostachys juncea andP. huashanica (Poaceae) studied by banding techniques. — Pl. Syst. Evol.151: 203–213.Google Scholar
  17. —, —,Jacobsen, N., 1980: Giemsa C-banding in Asiatic taxa ofHordeum sectionStenostachys with notes on chromosome morphology. — Hereditas93: 235–254.Google Scholar
  18. —, 1989: Giemsa C-banded karyotypes of three subspecies ofTaeniatherum caput-medusae and of two intergeneric hybrids withPsathyrostachys spp. (Poaceae). — Hereditas110: 283–288.Google Scholar
  19. Löve, A., 1984: Conspectus of theTriticeae. — Feddes Repert.95: 425–521.Google Scholar
  20. —, 1986: Some taxonomical adjustments in Eurasiatic wheatgrasses. — Veröff. Geobot. Inst. ETH, Stiftung Rübel, Zürich,87: 43–52.Google Scholar
  21. Morris, K. L. D., Gill, B. S., 1987: Genomic affinities of individual chromosomes based on C- and N-banding analyses of tetraploidElymus species and their diploid progenitor species. — Genome29: 247–252.Google Scholar
  22. Mukai, Y., Endo, T. R., Gill, B. S., 1991: Physical mapping of the 18S.26S rRNA multigene family in common wheat: identification of a new locus. — Chromosoma100: 71–78.Google Scholar
  23. Pedersen, C., Linde-Laursen, I., 1994: Chromosomal locations of four minor rDNA loci and a marker microsatellite sequence in barley. — Chromosome Res.2: 65–71.PubMedGoogle Scholar
  24. —, 1996: Genome and chromosome identification in cultivated barley and related species of theTriticeae (Poaceae) by in situ hybridization with the GAA-satellite sequence. — Genome39: 93–104.PubMedGoogle Scholar
  25. Seberg, O., Frederiksen, S., Baden, C., Linde-Laursen, I., 1991:Peridictyon, a new genus from the Balkan peninsula, and its relationship withFestucopsis (Poaceae). — Willdenowia21: 87–104.Google Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Ib Linde-Laursen
    • 1
  • Ole Seberg
    • 2
  • Signe Frederiksen
    • 2
  • Claus Baden
    • 3
  1. 1.Environmental Science and Technology Department, Plant GeneticsRisø National LaboratoryRoskildeDenmark
  2. 2.Botanical Institute, Botanical LaboratoryUniversity of CopenhagenCopenhagen KDenmark
  3. 3.Botanical SectionThe Royal Veterinary and Agricultural UniversityFrederiksberg C (Copenhagen)Denmark

Personalised recommendations