Cereal leaf beetle response to biochemicals from barley and pea seedlings. I. Crude extract, hydrophobic and hydrophilic fractions
Abstract
To identify contact feeding stimulants for adult cereal leaf beetles (CLB),Oulema melanopus (L.), compounds extracted from a host plant,Hordeum vulgare L. (cultivar ‘Lakeland’), were bioassayed in 3% agar. Damage to agar gel with the hydrophilic fraction was dominated by narrow channels; the hydrophobic fraction gel was dominated by bites, rashes, and shorter channels. This difference probably reflected the functional nature of compounds in each fraction. Crude extract and the hydrophobic fraction of nonhost pea seedlings,Pisum sativum L., prevented CLB feeding behavior, but the hydrophilic fraction only reduced it. Combination of barley and pea crude extracts, by weight at 1∶1 or greater, evoked a small feeding response which indicated a host-specific recognition factor in the barley.
Key words
Cereal leaf beetle barley feeding stimulants bioassay Oulema melanopus Hordeum vulgarePreview
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