Plant Systematics and Evolution

, Volume 213, Issue 1–2, pp 43–55

RAPD characterisation of two neotropical hybrid legumes

  • J. A. Hawkins
  • S. A. Harris
Article

Abstract

An investigation of randomly amplified polymorphic DNA (RAPD) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) marker distribution was made for two well-characterised hybrids and their parents,Leucaena leucocephala andL. esculenta andParkinsonia aculeata andCercidium praecox. Three chloroplast DNA (cpDNA) markers identified the maternal parent of eachL. leucocephala ×L. esculenta hybrid. Fifteen species-diagnostic RAPD markers (invariant in one taxon and absent from the other) were always present in theLeucaena hybrid and assumed to be of nuclear origin, whilst three RAPD markers showed expression patterns identical to the cpDNA markers and were assumed to be of organellar origin. No RAPD or PCR-RFLP taxon-diagnostic markers were discovered for eitherP. aculeata orC. praecox. However, 21 RAPD markers were species-specific (polymorphic within one taxon but absent from the other) and Southern analysis indicated that none of the markers were of organellar origin. Only 67% additivity of markers specific toP. aculeata andC. praecox was demonstrated in the hybrids between these two species, whilst inLeucaena 97% additivity was demonstrated. Differences between the two hybridising situations were related to the behaviour of the molecular markers and the biology of the species.

Key words

Leucaena leucocephala L. esculenta Parkinsonia aculeata Cercidium praecox RAPDs hybridisation RFLP-PCR markers 

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Copyright information

© Springer-Verlag 1998

Authors and Affiliations

  • J. A. Hawkins
    • 1
  • S. A. Harris
    • 1
  1. 1.Department of Plant SciencesUniversity of OxfordOxfordEngland, UK

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