A quantitative feeding bioassay forPissodes strobi Peck (Coleoptera: Curculionidae)
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A feeding bioassay forPissodes strobi Peck was developed that consisted of plastic petri dishes containing paired agar disks immersed in paraffin wax. Lens paper covering the top surface of the disks provided 1, 2, or 3 weevils in each replicate with a surface through which they made regular feeding punctures which could be easily counted. The number of feeding punctures was correlated with the amount of agar ingested. Candidate feeding stimulants or deterrents were applied to the paper covering one of the disks in the dishes, while the other served as a solvent control. Feeding stimulants were tested using disks of pure agar, and deterrents were assayed on agar disks that contained 2% ground, dried Sitka spruce bark,Picea sitchensis Carr. The weevils exhibited a concentration-dependent response to the amount of spruce bark in the agar disk. Feeding stimulation by Sitka spruce bark extracts, and deterrency by leaf oil of western red cedar,Thuja plicata Donn, was demonstrated. The bioassay would be useful in chemical isolation programs aimed at identification of feeding stimulants and deterrents.
Key wordsPissodes strobi Coleoptera Curculionidae picea sitchensis Carr. Thuja plicata Donn feeding bioassay feeding deterrents feeding stimulants
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- Anderson, J.M., andFisher, K.C. 1956. Repellency and host specificity in the white pine weevil (Pissodes strobi).Physiol Zool. 29:314–324.Google Scholar
- Anderson, J.M., andFisher, K.C. 1960. The response of white pine weevil to naturally occurring repellents.Can. J. Zool. 38:58–67.Google Scholar
- Hedin, P.A., Maxwell, F.G., andJenkins, J.N. 1974. Insect plant attractants, feeding stimulants, repellents, deterrents and other related factors affecting insect behavior, pp. 494–527,in F.G. Maxwell and F.A. Harris (eds.). Proceedings of the Summer Institute on Biological Control of Plant Insects and Diseases. University of Mississippi Press, Jackson, Mississippi. 647 pp.Google Scholar
- Keller, J.C., Maxwell, F.G., andJenkins, J.N. 1962. Cotton extracts as arrestants and feeding stimulants for the boll weevil.J. Econ. Entomol. 55:800.Google Scholar
- Munakata, K. 1977. Insect feeding deterrents in plants, pp. 93–102,in H.H. Shorey and J.J. McKelvey (eds.). Chemical Control of Insect Behaviour. John Wiley & Sons, New York. 414 pp.Google Scholar
- Peacock, J.W., Kennedy, B.H., andFisk, F.W. 1967. A bioassay technique for elm bark beetle feeding stimulants.Ann. Entomol. Soc. Am. 60:480–481.Google Scholar
- VanderSar, T.D.J., andBorden, J.H. 1977a. Visual orientation ofPissodes strobi Peck (Coleoptera:Curculionidae) in relation to host selection behaviour.Can. J. Zool. 55: 2042–2049.Google Scholar
- VanderSar, T.D.J., andBorden, J.H. 1977b. Aspects of host selection behaviour ofPissodes strobi Peck (Coleoptera: Curculionidae) as revealed in laboratory feeding bioassays.Can. J. Zool. 55:405–414.Google Scholar
- Zerillo, R.T., andOdell, T.M. 1973. White pine weevil (Pissodes strobi Peck): a rearing procedure and artificial medium.J. Econ. Entomol. 66:593–594.Google Scholar