Plant Systematics and Evolution

, Volume 214, Issue 1–4, pp 147–160 | Cite as

Molecular phylogeny ofCheirolophus (Asteraceae:Cardueae-Centaureinae) based on ITS sequences of nuclear ribosomal DNA

  • Alfonso Susanna
  • Teresa Garnatje
  • Núria Garcia-Jacas


The genusCheirolophus has an interesting western Mediterranean and Macaronesian distribution. Here we investigate the delimitation of the genus and its exclusion from the large genusCentaurea, the systematic position of the related genusPaleocyanus, the delimitation of some species and the phylogeny of the group. We have carried out a phylogenetic analysis of the PCR-generated sequences of the internal transcribed spacers (ITS-1 and ITS-2) of the nuclear ribosomal DNA. The results suggest that the genus, includingPaleocyanus crassifolius is monophyletic; thus, a new combination of this species underCheirolophus is proposed. The Macaronesian group of species is also monophyletic, indicating a single colonization of the archipelago. The poor resolution of microspecies in the Macaronesian group reinforces the hypothesis of a very recent differentiation of the group.

Key words

Asteraceae Cardueae-Centaureinae Cheirolophus Mediterranean molecular phylogeny ITS 


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  1. Baldwin, B. G., 1992: Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: an example from theCompositae. — Molec. Phylogenet. Evol.1: 3–16.Google Scholar
  2. —, 1993: Molecular phylogenetics ofCalycadenia (Compositae) based on ITS sequences of nuclear ribosomal DNA: chromosomal and morphological evolution reexamined. — Amer. J. Bot.80: 222–238.Google Scholar
  3. Bayer, R. J., Soltis, D. E., Soltis, P. S., 1996: Phylogenetic inferences inAntennaria (Asteraceae:Gnaphalieae:Cassiniinae) based on sequences from nuclear ribosomal DNA internal transcribed spacers (ITS). — Amer. J. Bot.83: 516–527.Google Scholar
  4. Boissier, E., 1839–1845: Voyage botanique dans le midi de l'Espagne pendant l'année 1837. — Paris.Google Scholar
  5. Bolòs, O., 1958: Grupos corológicos de la flora balear. — Publ. Inst. Biol. Apl. Barcelona27: 49–71.Google Scholar
  6. Bramwell, D., 1976: The endemic flora of the Canary Islands. Distribution, relationships and phytogeography. — InKunkel, G., (Ed.): Biogeography and ecology in the Canary Islands, pp. 207–240. — The Hague: Junk.Google Scholar
  7. —, (Ed.), 1979: Plants and islands. — London: Academic Press.Google Scholar
  8. Bremer, K., 1988: The limits of amino acid sequence data in angiosperm phylogenetic reconstruction. — Evolution42: 795–803.Google Scholar
  9. —, 1994:Asteraceae. Cladistics and classification. — Portland: Timber Press.Google Scholar
  10. Carlquist, S., 1976: Tribal interrelationships and phylogeny of theAsteraceae. — Aliso8: 465–492.Google Scholar
  11. Cassini, H., 1817:Cheirolophus. — Dictionnaire de Sciences Naturelles1, pp. 250. — Paris.Google Scholar
  12. Dittrich, M., 1968: Karpologische Untersuchungen zur Systematik vonCentaurea und verwandten Gattungen. — Bot. Jahrb. Syst.88: 70–162.Google Scholar
  13. —, 1977:Cynareae — systematic review. — InHewywood, H. W., Harborne, J. B., Turner, B. L., (Eds): The biology and chemistry ofCompositae. — London, New York: Oriole.Google Scholar
  14. Donoghue, M. J., Olmstead, R. G., Smith, J. F., Palmer, J. D., 1992: Phylogenetic relationships ofDipsacales based onrbcL sequences. — Ann. Missouri Bot. Gard.79: 333–345.Google Scholar
  15. Dostál, J., 1971: Preliminary notes on the subtribeCentaureinae. — Acta Bot. Acad. Sci. Hung.19: 73–79.Google Scholar
  16. —, 1976:Centaurea. — InTutin, T. G., Heywood, V. H., Burges, N. A., Valentine, D. H., Walters, S. M., Webb, D. A., (Eds): Flora Europaea,4, pp. 254–301. — Cambridge: Cambridge University Press.Google Scholar
  17. Doyle, J. J., Doyle, J. L., 1987: A rapid DNA isolation procedure for small quantities of fresh leaf tissue. — Phytochem. Bull.19: 11–15.Google Scholar
  18. Felsenstein, J., 1985: Confidence limits on phylogenies: an approach using the bootstrap. — Evolution39: 783–791.Google Scholar
  19. Fiori, A., Paoletti, G., 1896–1909: Flora Analitica d'Italia. — Padova.Google Scholar
  20. Font Quer, P., 1927: La flora de las Pitiusas y sus afinidades con la de la Penánsula Ibérica. — Mem. Real Acad. Ci. Barcelona20: 109–154.Google Scholar
  21. Francisco-Ortega, J., Jansen, R. K., Santos-Guerra, A., 1996: Chloroplast DNA evidence of colonization, adaptive radiation, and hybridization in the evolution of the Macaronesian flora. — Proc. Natl. Acad. Sci. USA93: 4085–4090.Google Scholar
  22. Garnatje, M. T., 1995: Relació entre el polimorfisme enzimàtic i alguns aspectes de l'especiació i de l'evolució en el gènereCheirolophus Cass. — Doctoral Dissertation, Universitat Autònoma de Barcelona.Google Scholar
  23. —, 1998: Isozyme studies in the genusCheirolophus Cass. (Asteraceae:Cardueae-Centaureinae) in the Iberian Peninsula, North Africa and the Canary Islands. — Pl. Syst. Evol.213: 57–70.Google Scholar
  24. Maddison, D. R., 1991: The discovery and importance of multiple islands of most parsimonious trees. — Syst. Zool.40: 315–328.Google Scholar
  25. Mes, T. H. M., Van Brederode, J., 'T Hart, H. T., 1996: Origin of the woody MacaronesianSempervivoideae and the phylogenetic position of the east African species ofAeonium. — Bot. Acta109: 477–491.Google Scholar
  26. Morgan, D. R., 1997: Decay analysis of large sets of phylogenetic data. — Taxon46: 509–517.Google Scholar
  27. —, 1994: Systematic and evolutionary implications ofrbcL sequence variation inRosaceae. — Amer. J. Bot.81: 890–903.Google Scholar
  28. Nepokroeff, M., Sytsma, K. J., 1996: Systematics and patterns of speciation and colonization in HawaiianPsychotria and relatives based on phylogenetic analysis of ITS sequence data. — Amer. J. Bot.83, Suppl.: 181–182.Google Scholar
  29. Olivares, A., Stübing, G., Peris, J. B., Martín, J., 1995:Cheirolophus lagunae sp. nov. (Asteraceae), endemismo iberolevantino. — Anales Jard. Bot. Madrid53: 262–265.Google Scholar
  30. Sang, T., Crawford, D. J., Kim, S.-C., Stuessy, T. F., 1994: Radiation of the endemic genusDendroseris (Asteraceae) on the Juan Fernández islands: evidence from sequences of the ITS regions of nuclear ribosomal DNA. — Amer. J. Bot.81: 1494–1501.Google Scholar
  31. —, 1995: ITS sequences and phylogeny of the genusRobinsonia (Asteraceae). — Syst. Bot.20: 55–64.Google Scholar
  32. Soltis, D. E., Kuzoff, R. K., 1993: ITS sequence homogeneity within and among populations ofLomatium grayi andL. laevigatum (Umbelliferae). — Molec. Phylogenet. Evol.2: 166–170.Google Scholar
  33. —, 1991: TheHeuchera group (Saxifragaceae): evidence for chloroplast transfer and paraphyly. — Amer. J. Bot.78: 1091–1112.Google Scholar
  34. Stübing, G., Peris, J. B., Olivares, A., Martín, J., 1997:Cheirolophus mansanetianus Stübing, Peris, Olivares & Martín, sp. nov. andCh. grandifolius (Font Quer) comb. & stat. nov. (Asteraceae), two endemics from Spain. — Anales Jard. Bot. Madrid55: 170–173.Google Scholar
  35. Susanna, A., 1989: Mapa 114.Cheirolophus intybaceus (Lam.)Dostal. — InFernández Casas, J., (Ed.): Asientos para un atlas corológico de la flora occidental, 11. — Fontqueria22: 16–18.Google Scholar
  36. —, 1991: Mapa 478.Cheirolophus sempervirens (L.)Pomel. — InFernández Casas, J., Gamarra, R., (Eds): Asientos para un atlas corológico de la flora occidental, 18. Fontqueria31: 267–269.Google Scholar
  37. —, 1993: Mapa 511.Cheirolophus uliginosus (Brot.)Dostál. — InFernández Casas, J., Morales, M. J., (Eds): Asientos para un atlas corológico de la flora occidental. — Fontqueria36: 208–210.Google Scholar
  38. —, 1995: Phylogenetic relationships in tribeCardueae (Asteraceae) based on ITS sequences. — Amer. J. Bot.82: 271–294.Google Scholar
  39. Swofford, D. L., 1991: PAUP: phylogenetic analysis using parsimony, version 3.1. — Champaign: Illinois Natural History Survey.Google Scholar
  40. —, 1990: Phylogeny reconstruction. — InHillis, D., Moritz, C., (Eds): Molecular systematics, pp. 411–501. — Sunderland: Sinauer.Google Scholar
  41. Takhtajan, A., 1986: Floristic regions of the world. — Berkeley: California University Press.Google Scholar
  42. Wagenitz, G., 1955: Pollenmorphologie und Systematik in der GattungCentaurea L. s. l. — Flora142: 213–279.Google Scholar
  43. —, 1974: Parallele Evolution von Merkmalen in der GattungCentaurea. — Phyton (Austria)16: 301–312.Google Scholar
  44. —, 1982:Centaurothamnus, eine neue Gattung derCompositae-Cardueae aus Arabien. — Candollea37: 101–115.Google Scholar
  45. White, T. J., Bruns, T., Lee, S., Taylor, J., 1990: Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. — InInnis, M., Gelfand, D., Sninsky, J., White, T., (Eds): PCR protocols: a guide to methods and applications, pp. 315–322. — San Diego: Academic Press.Google Scholar
  46. Wojciechowski, M. F., Sanderson, M. J., Baldwin, B. G., Donoghue, M. J., 1993: Monophyly of aneuploidAstragalus (Fabaceae): evidence from nuclear ribosomal DNA Internal transcribed sequences. — Amer. J. Bot.80: 711–722.Google Scholar

Copyright information

© Springer-Verlag 1999

Authors and Affiliations

  • Alfonso Susanna
    • 1
  • Teresa Garnatje
    • 1
  • Núria Garcia-Jacas
    • 1
  1. 1.Institut Botànic de Barcelona (C.S.I.C.)BarcelonaSpain

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