Genome size variation in Cajanus cajan ( Fabaceae): A reconsideration Article Received: 18 February 1997 Revised: 02 June 1997 Accepted: 04 June 1997 DOI:
Cite this article as: Greilhuber, J. & Obermayer, R. Pl Syst Evol (1998) 212: 135. doi:10.1007/BF00985225 Abstract
A recent investigation of genome size in certain samples of the pigeonpea,
Cajanus cajan, indicates values from 1.55 pg to 1.99 pg (1C level), which is 1.29-fold variation between accessions. In the present analysis those of these accessions which had particularly high or low DNA contents in that study were subjected to a reanalysis using propidium iodide and DAPI flow cytometry and Feulgen densitometry. Only minor differences in genome size, not more than 1.047-fold, were found with flow cytometry, and no significant differences were obtained with Feulgen densitometry. The previously reported genome size cannot be confirmed. It is about half as large and was determined in the present study as 0.825 pg (1C, propidium iodide flow cytometry, Glycine max as standard) and 0.853 pg (1C, Feulgen densitometry, Allium cepa and Pisum sativum as standards), respectively. Key words Fabaceae Cajanus cajan Genome size variation flow cytometry propidium iodide DAPI Feulgen densitometry References
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