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Plant Systematics and Evolution

, Volume 210, Issue 1–2, pp 41–50 | Cite as

Comparative karyotype analysis ofCeratozamia mexicana andMicrocycas calocoma (Zamiaceae) using fluorochrome banding (CMA/DAPI) and fluorescence in situ hybridization of ribosomal DNA

  • Goro Kokubugata
  • Katsuhiko Kondo
Article

Abstract

Orcein staining, differential staining with CMA and DAPI, and FISH with an rDNA probe were used to compare somatic chromosomes ofCeratozamia mexicana andMicrocycas calocoma. CMA-positive dots and hybridization signals appeared on chromosomes at early interphase and mitotic prophase, but in significantly different number in the two species. InCeratozamia mexicana, the CMA-positive and DAPI-negative bands and the hybridization signal were located at the terminal region of the long arm of three median-centromeric chromosomes, the terminal region of the short arm of two median-centromeric chromosomes and the terminal region of the long arm of two subterminal-centromeric chromosomes. InMicrocycas calocoma, they were located at the pericentric region of two median-centromeric chromosomes. These chromosome data suggested thatMicrocycas has no simple Robertsonian relationship toCeratozamia.

Key words

Zamiaceae Ceratozamia Microcycas CMA DAPI FISH karyotype 

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Copyright information

© Springer-Verlag 1998

Authors and Affiliations

  • Goro Kokubugata
    • 1
  • Katsuhiko Kondo
    • 1
  1. 1.Laboratory of Plant Chromosome and Gene StockFaculty of Science, Hiroshima UniversityHigashi-HiroshimaJapan

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