Skip to main content
Log in

RAPD variations among pure and hybrid populations ofPicea mariana, P. rubens, andP. glauca (Pinaceae), and cytogenetic stability ofPicea hybrids: Identification of species-specific RAPD markers

  • Published:
Plant Systematics and Evolution Aims and scope Submit manuscript

Abstract

Random amplified polymorphic DNA (RAPD) analysis was used to determine genetic relationships amongP. mariana (black spruce),P. rubens (red spruce), andP. glauca (white spruce) and to assess the degree of polymorphism within populations from different provenances and among spruce hybrids. Eleven arbitrary decamer primers were used to amplify genomic DNAs extracted from embryogenic cultures and seedlings. Species-specific RAPD markers were identified.Picea mariana andP. rubens showed similar RAPD profiles confirming their close genetic relationship. Species-specific RAPD markers were identified and were useful in distinguishing white spruce from black and red spruces. RAPD differentiation between populations within each species was small. The level of polymorphism was much higher in spruce hybrid populations than in the pure species. Cytological analysis ofP. mariana ×P. rubens hybrids showed normal mitotic behaviour at prophase, metaphase, anaphase, and telophase. All the hybrids analyzed from different cross combinations were euploids.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bobola, M. S., Eckert, R. T., Klein, A. S., 1992a: Restriction fragment variation in nuclear ribosomal DNA repeat unit within and betweenPicea rubens andPicea mariana. — Canad. J. Forest Res.22: 255–263.

    Google Scholar 

  • Smith, D. E., Klein, A. S., 1992b: Five major nuclear ribosomal repeats represent a large and variable fraction of the genomic DNA ofPicea rubens andP. mariana. — Molec. Biol. Evol.9: 125–137.

    Google Scholar 

  • Cheliak, W. M., Klimaszewska, K., 1991: Genetic variation in somatic embryogenic response in open-pollinated families of black spruce. — Theor. Appl. Genet.82: 185–190.

    Google Scholar 

  • Gordon, A. G., 1976: The taxonomy and genetics ofPicea rubens and its relationship toPicea mariana. — Canad. J. Bot.54: 781–813.

    Google Scholar 

  • Isabel, N., Tremblay, L., Michaud, M., Tremblay, F. M., Bousquet, J., 1993: RAPDs as an aid to evaluate the genetic integrity of somatic embryogenesis-derived populations ofPicea mariana (Mill) B. S. P. — Theor. Appl. Genet.86: 81–87.

    Google Scholar 

  • King, J. N., Dhir, N. K., Dancik, B. P., 1984: Genetic structure and mating system of white spruce (Picea glauca) in a seed production area. — Canad. J. Forest Res.14: 639–643.

    Google Scholar 

  • Liu, Z., Furnier, G. R., 1993: Comparison of allozyme, RFLP, and RAPD markers for revealing genetic variation within and between trembling aspen and bigtooth aspen. — Theor. Appl. Genet.87: 97–105.

    Google Scholar 

  • Lukaszewski, A. J., Gustafson, J. P., 1983: Translocation and modifications of chromosomes in triticale × wheat hybrids. — Theor. Appl. Genet.64: 239–248.

    Google Scholar 

  • Maniatis, T., Fritsch, E. F., Sambrook, J., 1989: Molecular cloning: a laboratory manual. 2nd edn,1-3 — Long Island, N.Y.: Cold Spring Harbor Laboratory.

    Google Scholar 

  • Manley, S. A. M., 1971: Identification of red, black, and hybrid spruces. — Canad. Dep. Fish. Forest. Canad. Forest Serv. Publ.1301.

  • Mosseler, A., Egger, K. N., Hughes, G. A., 1992: Low level of genetic diversity in red pine confirmed by random amplified polymorphic DNA markers. — Canad. J. Forest Res.22: 1332–1337.

    Google Scholar 

  • Murray, M. G., Thompson, W. F., 1980: Rapid isolation of high molecular weight plant DNA. — Nucl. Acids Res.8: 4321–4325.

    Google Scholar 

  • Nkongolo, K. K., 1996: Chromosome analysis and DNA homology in threePicea species,P. mariana, P. rubens, andP. glauca (Pinaceae). — Pl. Syst. Evol.203: 27–40.

    Google Scholar 

  • , 1994: Karyotype analysis and optimization of mitotic index inPicea mariana (black spruce) preparations from seedling root tips and embryogenic cultures. — Heredity73: 195–203.

    Google Scholar 

  • , 1995: Cytological and molecular characterization ofLarix decidua, L. leptolepis, andL. ×eurolepis: identification of species specific chromosomes and enhancement of mitotic index. — Theor. Appl. Genet.90: 827–834.

    Google Scholar 

  • , 1996: Genetic and cytogenetic of Russian wheat aphid resistance in triticale × wheat hybrids and progenies. — Crop. Sci.36: 1114–1119.

    Google Scholar 

  • -Klimaszewska, K., Gratton, W., Michael, P., 1998: Optimization of RAPD patterns and DNA yields using embryogenic lines and seedlings. — Plant Mol. Biol. Rep.16 (3) (in press).

  • Singh, R. J., 1993: Plant cytogenetics. — Boca Raton, Fl.: CRC Press.

    Google Scholar 

  • Von Rudloff, E., 1966: Gas-liquid chromatography of terpene. Part XIV. The Chemical composition of the volatile oil of the leaves ofPicea rubens Sag. and chemotaxonomic correlations with North American spruce species. — Phytochemistry5: 331–341.

    Google Scholar 

  • Wilkinson, R. C., 1970: Chemical evidence of species relationships for northeastern North American spruces. — Ph.D. Thesis, Michigan State University, East Lansing, Michigan.

    Google Scholar 

  • Williams, J. G. K., Kublelik, A. R., Livak, K. J., Rafalski, J. A., Tingey, S. V., 1990: DNA polymorphisms amplified by arbitrary primer 774s are useful as genetic markers. — Nucl. Acids Res.18: 6531–6535.

    Google Scholar 

  • Yeh, F. C., Khali, M. A. K., El-Kassaby, Y. A., Trust, D. C., 1986: Allozyme variation inPicea mariana from Newfoundland: genetic diversity, population structure, and analysis of differentiation. — Canad. J. Forest Res.16: 713–720.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nkongolo, K.K. RAPD variations among pure and hybrid populations ofPicea mariana, P. rubens, andP. glauca (Pinaceae), and cytogenetic stability ofPicea hybrids: Identification of species-specific RAPD markers. Pl Syst Evol 215, 229–239 (1999). https://doi.org/10.1007/BF00984657

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00984657

Key words

Navigation