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Plant Systematics and Evolution

, Volume 215, Issue 1–4, pp 85–99 | Cite as

The use of a non-coding region of chloroplast DNA in phylogenetic studies of the subtribeSonchinae (Asteraceae:Lactuceae)

  • Seung-Chul Kim
  • Daniel J. Crawford
  • Robert K. Jansen
  • Arnoldo Santos-Guerra
Article

Abstract

The systematic utility of sequences from a non-coding region of chloroplast DNA (cpDNA) betweenpsbA andtrnH(GUG) was examined by assessing phylogenetic relationships in subtribeSonchinae (Asteraceae:Lactuceae). Primers constructed against highly conserved regions of tRNA genes were used for PCR amplification and sequencing. ThepsbA-trnH intergenic spacer contains several insertions and deletions (indels) inSonchinae with the length varying from 385 to 450 bp. Sequence divergence ranges from 0.00% to 7.54% withinSonchinae, with an average of 2.4%. Average sequence divergence inSonchus subg.Sonchus is 2.0%, while the mean for subg.Dendrosonchus and its close relatives in Macaronesia (the woodySonchus alliance) is 1.0%. Our results suggest that this region does not evolve rapidly enough to resolve relationships among closely related genera or insular endemics in theAsteraceae. The phylogenetic utility ofpsbA-trnH sequences of the non-coding cpDNA was compared to sequences from the ITS region of nuclear ribosomal DNA. The results suggest that ITS sequences evolve nearly four times faster thanpsbA-trnH intergenic spacer sequences. Furthermore, the ITS sequences provide more variable and phylogenetically informative sites and generate more highly resolved trees with more strongly supported clades, and thus are more suitable for phylogenetic comparisons at lower taxonomic levels than thepsbA-trnH intergenic chloroplast sequences.

Key words

Sonchinae Asteraceae cpDNA non-coding region (psbA-trnHGUGphylogeny 

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Copyright information

© Springer-Verlag 1999

Authors and Affiliations

  • Seung-Chul Kim
    • 1
  • Daniel J. Crawford
    • 1
  • Robert K. Jansen
    • 2
  • Arnoldo Santos-Guerra
    • 3
  1. 1.Department of Plant BiologyThe Ohio State UniversityColumbusUSA
  2. 2.Department of Botany and Institute of Cell and Molecular BiologyThe University of TexasAustinUSA
  3. 3.Jardín de Aclimatación de La OrotavaPuerto de la Cruz, TenerifeSpain

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