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Journal of Chemical Ecology

, Volume 19, Issue 11, pp 2521–2541 | Cite as

Aggregation arrestant pheromone of the German cockroach,Blattella germanica (L.) (Dictyoptera: Blattellidae): Isolation and structure elucidation of blattellastanoside-A and -B

  • Masayuki Sakuma
  • Hiroshi Fukami
Article

Abstract

The aggregation pheromone of the German cockroach,Blattella germanica, consists of attractant and arrestant, which can be detected by olfactometer and choice-chamber assay, respectively. Both were extracted from the frass-contaminated filter paper being used as a shelter. They were separated by solvent partition withn-butanol and water. The arrestant from then-butanol phase was purified by open column chromatography and then successive HPLC isolated two major arrestant components. Spectral evidence from SI-MS, HR-EI-MS, and NMR experiments with pulse techniques provided possible structures as 1-(6α-chloro-4β,5β-epoxy-5β-stigmast-3β-yl)-β-d-glucopyranoside and 1-(6α-chloro-5β-hydroxy-5β-stigmast-3β-yl)-β-d-glu-copyranoside, denoted as blattellastanoside-A and blattellastanoside-B, respectively. They represented arrestant activity as median effective doses (ED50) at 0.044 (A) and 3.2 (B) nmol on 1.0 cm2 of Whatman No. 1 filter paper.

Key words

Dictyoptera Blattellidae Blattella germanica German cockroach cockroach arrestant orientation pheromone aggregation pheromone steroid blattellastanoside 

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Copyright information

© Plenum Publishing Corporation 1993

Authors and Affiliations

  • Masayuki Sakuma
    • 1
  • Hiroshi Fukami
    • 1
  1. 1.Pesticide Research Institute Faculty of AgricultureKyoto UniversityKyotoJapan

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