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Neurochemical Research

, Volume 19, Issue 11, pp 1413–1419 | Cite as

Fast and slow axonal transport-different methodological approaches give complementary information: contributions of the stop-flow/crush approach

  • Annica B. Dahlström
  • Jia-Yi Li
Original Articles

Abstract

This “minireview” describes experiments in short term crush operated rat nerves, to study endogenous substances in anterograde and retrograde fast axonal transport. Immunofluorescence was used to recognize transported antigens, and cytofluorimetric scanning was employed to quantitate different antigens which had accumulated proximal and distal to the crushes. Vesicle membrane components p38 (synaptophysin) and SV2 accumulated on both sides of a crush. This was expected from a number of studies from different laboratories. Surface associated molecules, however, like synapsins and rab3a, have been studied by other groups with biochemical methods, and suggested to be transported with slow transport. The crush method, however, revealed that a considerable fraction of these two substances are transported with the fast transport system, and, thus, associated with fast transported organelles in the living neuron. Evidently, more than one technique is required to give a more complete picture of intraneuronal transport related events.

Key Words

Axonal transport synaptophysin synapsin I Rab3a immunofluorescence cytofluorimetric scanning confocal laser scanning 

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Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • Annica B. Dahlström
    • 1
  • Jia-Yi Li
    • 2
  1. 1.Department of Anatomy and Cell BiologyUniversity of GöteborgGöteborgSweden
  2. 2.Department of AnatomyLuzhou Medical CollegeLuzhou, SichuanP. R. China

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