Neurochemical Research

, Volume 19, Issue 3, pp 339–345 | Cite as

The ontogeny of apomorphine-induced alterations of neostriatal dopamine release: Effects on potassium-evoked release

  • Russell A. Gazzara
  • Susan L. Andersen
Original Articles
Accepted:

Abstract

The effects of apomorphine (0.05, 0.1, and 1.0 mg/kg, s.c.) on K+-evoked dopamine release were studied through the use of in vivo microdialysis in the neostriatum of developing and adult rats. Fifteen-minute samples were collected from urethane-anesthetized rats 5, 10–11, 21–22, 35–36 days of age, and adults, and quantified by high performance liquid chromatography with electrochemical detection. Apomorphine attenuated K+-evoked dopamine release in all age groups, suggesting that the dopamine autoreceptor modulating release in the neostriatum is functional by 5 days of age. A dose-response effect of apomorphine was observed in all age groups except at 5 and 10 days of age. Absolute levels of extracellular dopamine were significantly lower at 5 and 10 days of age compared with the other ages, and the effectiveness of a high-K+ artificial cerebrospinal fluid to evoke dopamine release increased with age.

Key Words

Dopamine development microdialysis apomorphine autoreceptor potassium 
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References

  1. 1.
    Bowyer, J. F., and Weiner, N. 1987. Modulation of the Ca2+-evoked release of [3H]dopamine from striatal synaptosomes by dopamine (D2) agonists and antagonists. J. Pharmacol. Exp. Ther. 241:27–33.PubMedGoogle Scholar
  2. 2.
    Starke, K., Reimann, W., Zumstein, A., and Hertting, G. 1978. Effect of dopamine receptor agonists and antagonists on release of dopamine in the rabbit caudate nucleus in vitro. Naunyn Schmiedebergs Arch. Pharmacol. 305:27–36.PubMedGoogle Scholar
  3. 3.
    Jackisch, R., Zumstein, A., Hertting, G., and Starke, K. 1980. Interneurons are probably not involved in the presynaptic dopaminergic control of dopamine release in rabbit caudate nucleus. Naunyn Schmiedebergs Arch. Pharmacol. 314:129–133.PubMedGoogle Scholar
  4. 4.
    Zetterstrom, T., and Ungerstedt, U. 1984. Effects of apomorphine on the in vivo release of dopamine and its metabolites, studied by brain dialysis. Eur. J. Pharmacol. 97:29–36.PubMedGoogle Scholar
  5. 5.
    Zetterstrom, T., Sharp, T., and Ungerstedt, U. 1986. Effect of dopamine D-1 and D-2 receptor selective drugs on dopamine release and metabolism in rat striatum in vivo. Naunyn Schmiedebergs Arch. Pharmacol. 334:117–124.PubMedGoogle Scholar
  6. 6.
    Raiteri, M., Cervoni, A. M., and del Carmine, R. 1978. Do presynaptic autoreceptors control dopamine release? Nature 274:706–708.PubMedGoogle Scholar
  7. 7.
    Andersen, S. L., and Gazzara, R. A. In Press. The ontogeny of apomorphine-induced alterations of neostriatal dopamine release: effects on spontaneous release. J. Neurochem.Google Scholar
  8. 8.
    Sherwood, N. M., and Timiras, P. S. 1970. A Stereotaxic Atlas of the Developing Rat Brain. University of California Press, Berkeley.Google Scholar
  9. 9.
    Paxinos, G., and Watson, C. 1982. The Rat Brain in Stereotaxic Coordinates. 2nd Edition, Academic Press, New York.Google Scholar
  10. 10.
    Gazzara, R. A., and Andersen, S. L. 1991. Characterization of neostriatal dopamine release as measured by microdialysis in developing and adult rats. Soc. Neurosci. Abstr. 21:264.Google Scholar
  11. 11.
    Winer, B. J. 1971. Statistical Principles in Experimental Design. 2nd Edition, McGraw-Hill Book Company, New York.Google Scholar
  12. 12.
    Siegel, S. 1956. Nonparametric Statistics for the Behavioral Sciences. McGraw-Hill Book Co., New York.Google Scholar
  13. 13.
    De Vries, T. J., Mulder, A. H., and Schoffelmeer, A. N. M. 1992. Differential ontogeny of functional dopamine and muscarinic receptors mediating presynaptic inhibition of neurotransmitter release and postsynaptic regulation of adenylate cyclase activity in rat striatum. Dev. Brain Res. 66:91–96.Google Scholar
  14. 14.
    Spear, L. P. 1979. The use of psychopharmacological procedures to analyse the ontogeny of learning and retention: issues and concerns. Pages 135–156,in Spear, N. E., and Campbell, B. A. (eds.), Ontogeny of Learning and Memory, Lawrence Erlbaum Associates, Hillsdale.Google Scholar
  15. 15.
    Pitts, D. K., Freeman, A. S., and Chiodo, L. A. 1990. Dopamine neuron ontogeny: electrophysiological studies. Synapse 6:309–320.PubMedGoogle Scholar
  16. 16.
    Tepper, J. M., Trent, F., and Nakamura, S. 1990. Postnatal development of the electrical activity of rat nigrostriatal dopaminergic neurons. Dev. Brain Res. 54:21–33.Google Scholar
  17. 17.
    Coyle, J. T., and Campochiaro, P. 1976. Ontogenesis of dopaminergic-cholinergic interactions in the rat striatum: a neurochemical study. J. Neurochem. 27:673–678.PubMedGoogle Scholar
  18. 18.
    Rao, P. A., Molinoff, P. B., and Joyce, J. N. 1991. Ontogeny of dopamine D1 and D2 receptor subtypes in rat basal ganglia: a quantitative autoradiographic study. Dev. Brain Res. 60:161–177.Google Scholar
  19. 19.
    Levi, G., Gallo, V., Ciotti, T., and Raiteri, M. 1979. GABA fluxes in presynaptic nerve endings from immature rats. J. Neurochem. 33:1043–1053.Google Scholar
  20. 20.
    Nomura, Y., Yotsumoto, I., and Segawa, T. 1981. Ontogenetic development of high potassium- and acetylcholine-induced release of dopamine from striatal slices of the rat. Dev. Brain Res. 1:171–177.Google Scholar
  21. 21.
    Carlson, C. G. 1992. Early postnatal changes in presynaptic potassium sensitivity. Dev. Brain Res. 68:183–191.Google Scholar
  22. 22.
    Spampinato, U., Girault, J.-A., Danguir, J., Savaki, H. E., Glowinski, J., and Besson, M.-J. 1986. Apomorphine and haloperidol effects on striatal3H-dopamine release in anesthetized, awake restrained and freely moving rats. Brain Res. Bull. 16:161–166.PubMedGoogle Scholar
  23. 23.
    Kelland, M. D., Chiodo, L. A., and Freeman, A. S. 1990. Anesthetic influences on the basal activity and pharmacological responsiveness of nigrostriatal dopamine neurons. Synapse 6:207–209.PubMedGoogle Scholar
  24. 24.
    Opacka-Juffry, J., Ahier, R. G., and Cremer, J. E. 1991. Nomifensine-induced increase in extracellular striatal dopamine is enhanced by isoflurane anaesthesia. Synapse 7:169–171.PubMedGoogle Scholar
  25. 25.
    Tepper, J. M., Creese, I., and Schwartz, D. H. 1991. Stimulusevoked changes in neostriatal dopamine levels in awake and anesthetized rats as measured by microdialysis. Brain Res. 559:283–292.PubMedGoogle Scholar
  26. 26.
    Westerink, B. H. C., Hofsteede, H. M., Damsma, G., and de Vries, J. B. 1988. The significance of extracellular calcium for the release of dopamine, acetylcholine and amino acids in conscious rats, evaluated by brain microdialysis. Naunyn Schmiedebergs Arch. Pharmacol. 337:373–378.PubMedGoogle Scholar
  27. 27.
    Westerink, B. H. C., de Boer, P., and Damsma, G. 1990. Dopamine-acetylcholine interaction in the striatum studied by microdialysis in the awake rat: some methodological aspects. J. Neurosci. Methods 34:117–124.PubMedGoogle Scholar
  28. 28.
    Moghaddam, B., and Bunney, B. S. 1989. Ionic composition of microdialysis perfusing solution alters the pharmacological responsiveness and basal outflow of striatal dopamine. J. Neurochem. 53:652–654.PubMedGoogle Scholar
  29. 29.
    De Boer, P., Damsma, G., Fibiger, H. C., Timmerman, W., de Vries, J. B., and Westerink, B. H. C. 1990. Dopaminergic-cholinergic interactions in the striatum: the critical significance of calcium concentrations in brain microdialysis. Naunyn Schmiedebergs Arch. Pharmacol. 342:528–534.PubMedGoogle Scholar
  30. 30.
    Timmerman, W., and Westerink, B. H. C. 1991. Importance of the calcium content infused during microdialysis for the effects induced by D2 agonists on the release of dopamine in the striatum of the rat. Neurosci. Lett. 131:93–96.PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • Russell A. Gazzara
    • 1
    • 2
  • Susan L. Andersen
    • 1
    • 2
  1. 1.Department of PsychologyState University of New York at BinghamtonBinghamton
  2. 2.Center for Developmental PsychobiologyState University of New York at BinghamtonBinghamton

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