Neurochemical Research

, Volume 20, Issue 7, pp 827–831 | Cite as

Alteration of opioid peptide concentrations in the rat pituitary following survivable closed head injury

  • Olga O. Grigoriants
  • Svetlana V. Pravdenkova
  • Bruce J. Andersen
  • Dominic M. Desiderio
Original Articles
Accepted:

Abstract

Concentration changes of methionine enkephalin-like immjnoreactivity (ME-li) and β-endorphin-like immunoreactivity (BE-li) in the rat pituitary following diffuse brain injury were studied. Closed head injury was induced by a weight-drop trauma device (450 g×2 m). The level of closed head injury used in this study altered the pituitary opioid peptide concentrations. The level of ME-li did not change in the experimental groups 3 hours, 10 hours, 24 hours, and 3 days after the trauma, but significantly increased by 34% 10 days after the trauma. BE-li remained constant 3 hours and 10 hours following the injury, increased by 48% at 24 hours, and remained at this level for 10 days after the trauma (44% at 3 days, and 40% at 10 days). The levels of ME-li and BE-li in the control sham-operated rats did not change during these times. The present measurements of BE-li and ME-li in the pituitary indicate that the opioid peptides that derive from two different neuropeptidergic systems, proopiomelanocortin (POMC) and preproenkephalin A, respectively, may participate in the pathophysiology of a closed head injury.

Key Words

Opioid peptide head injury HPLC RIA 
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Copyright information

© Plenum Publishing Corporation 1995

Authors and Affiliations

  • Olga O. Grigoriants
    • 1
  • Svetlana V. Pravdenkova
    • 4
  • Bruce J. Andersen
    • 4
  • Dominic M. Desiderio
    • 1
    • 2
    • 3
  1. 1.Charles B. Stout Neuroscience Mass Spectrometry LaboratoryThe University of TennesseeMemphis
  2. 2.The Department of NeurologyThe University of TennesseeMemphis
  3. 3.The Department of BiochemistryThe University of TennesseeMemphis
  4. 4.The Department of NeurosurgeryThe University of ArkansasLittle Rock

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