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Neurochemical Research

, Volume 16, Issue 12, pp 1303–1310 | Cite as

Characterization of an ATP diphosphohydrolase (EC 3.6.1.5) in synaptosomes from cerebral cortex of adult rats

  • Ana M. O. Battastini
  • João B. T. da Rocha
  • Claudia K. Barcellos
  • Renato D. Dias
  • João J. F. Sarkis
Original Articles

Abstract

Data from the literature have demonstrated that synaptosomal preparations from various sources can hydrolyze externally added ATP. Various authors characterized this activity as an ecto-ATPase. In the present report, we demonstrate that synaptosomal preparations obtained from the cerebral cortex of rats show ATPase activity that could not be dissociated from ADPase activity, suggesting that an ATP-diphosphohydrolase is involved in ATP and ADP hydrolysis. Furthermore, the ATP and ADP hydrolysis could not be attributed to associations of enzymes that could mimic an ATP-diphosphohydrolase because none of the following activities were detected in our assay conditions inorganic pyrophosphatase, adenylate kinase, or nonspecific phosphatases. A possible association between an ATPase and an ADPase was excluded on the basis of both the kinetics and much additional data on inhibitors, ion dependence, pH, etc. The present results demonstrate that in synaptosomal preparations from cerebral cortex an ATP-diphosphohydrolase is involved, at least in part, in ATP and ADP hydrolysis.

Key Words

ATP diphosphohydrolase apyrase ATPase ADPase neurotransmission 

Abbreviations

DCCD

dicyclohexylcarboiimide

EDTA

ethylenediaminetetraacetic acid

HEPES

N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid

Pi

inorganic phosphate

Enzymes

ATP

diphosphohydrolase, Apyrase (EC 3.6.1.5)

ATPase

ATP phosphohydrolase (EC 3.6.1.3) 5′-nucleotidase (EC 3.1.3.5) Hexokinase (EC 2.7.1.1) Glucose-6-phosphate dehydrogenase (EC 1.1.1.49) Adenylate kinase (EC 2.7.4.3) Inorganic pyrophosphatase (EC 3.6.1.1)

ATP

pyrophosphohydrolase (EC 3.6.1.8)

LDH

lactate dehydrogenase (EC 1.1.1.27)

SDH

succinate dehydrogenase (EC 1.3.1.6)

ACHE

acethylcholinesterase (EC 3.1.1.7)

G-6-Pase

glucose-6-phosphatase (EC 3.1.3.9)

NADPH

cytoehrome c oxidoreductase (NCR) (EC 1.6.2.4)

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Copyright information

© Plenum Publishing Corporation 1991

Authors and Affiliations

  • Ana M. O. Battastini
    • 1
  • João B. T. da Rocha
    • 2
  • Claudia K. Barcellos
    • 3
  • Renato D. Dias
    • 3
  • João J. F. Sarkis
    • 3
  1. 1.Departamento de Ciências FisiológicasFundacão Universidade do Rio GrandeRio GrandeBrasil
  2. 2.Setor de Bioquímica-Centro de Ciências Naturais e ExatasUniversidade Federal de Santa MariaSanta MariaBrasil
  3. 3.Departamento de Bioquimica, Institute de BiociênciasUniversidade Federal do Rio Grande do SulPorto AlegreBrasil

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