Neurochemical Research

, Volume 16, Issue 10, pp 1175–1185 | Cite as

Characterization of divalent cation-induced [3H]Acetylcholine release from EGTA-treated rat hippocampal synaptosomes

  • Thomas W. Vickroy
  • Cynthia J. Schneider
Original Articles
Accepted:

Abstract

Calcium-naive synaptosomes were used to assess the effects of divalent cations on [3H]acetylcholine release from rat hippocampal homogenates. Following equilibration with calcium-free buffer (containing 10μM EGTA), calcium reversibly increased [3H]acetylcholine efflux (up to five-fold) while causing no measurable efflux of lactate dehydrogenase. When substituted for calcium, strongtium and barium behaved similarly although barium exhibited three-fold greater efficacy. In the presence of elevated potassium, 4-aminopyridine or tetraethylammonium, the secretagogue efficacy of calcium (but not barium) was markedly increased. The release-promoting effects of both cations were inhibited by lanthanum, magnesium, cadmium, and ω-conotoxin but were insensitive to nifedipine and cobalt (both 10 μM). In addition, stimulation of muscarnic cholinergic autoreceptors substantially inhibited both calcium and barium-evoked [3H]acetylcholine release. Taken together, these results indicate that cation-evoked transmitter release from calcium-naive synaptosomes is subject to normal neuroregulatory mechanisms and therefore should be useful for investigating presynaptic modulation of neuronal exocytosis.

Key Words

Acetylcholine barium calcium EGTA synaptosomes cation channels 
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Copyright information

© Plenum Publishing Corporation 1991

Authors and Affiliations

  • Thomas W. Vickroy
    • 1
  • Cynthia J. Schneider
    • 1
  1. 1.Departments of Physiological SciencesUniversity of FloridaGainesville

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