Neurochemical Research

, Volume 3, Issue 4, pp 411–422 | Cite as

Axonal transport of 4S RNA in the chick optic system

  • Suzanne Por
  • P. W. Gunning
  • P. L. Jeffrey
  • L. Austin
Original Articles

Abstract

The axonal transport of tRNA has been investigated in the chick optic system. Chicks were injected with [3H]uridine intraocularly or intracranially and the RNA of the retina, nerve complex, and tecta separated by polyacrylamide gel electrophoresis and then counted. The ratio of TRNA to rRNA specific activities increased with time in both the nerve complex and contralateral tectum. The ratio increased more rapidly in the nerve complex than the tectum. However, no increase was observed in the case of intracranially injected animals. This is consistent with the axonal flow of tRNA. When [methyl-3H]methionine was used as precursor, the preferential labeling of 4S RNA to rRNA which resulted more clearly showed a transport of 4S RNA from the retinal cells to the tectum. In conclusion, it was found that about 40% of the radioactive RNA observed within the optic tectum 4 days after an intraocular injection of [3H]uridine was accounted for by 4S RNA which had flowed from the retina. However, the migration of a methylated RNA molecule of size 4S, but unrelated to tRNA, cannot be entirely eliminated.

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References

  1. 1.
    Jeffrey, P. L., andAustin, L. 1973. Axoplasmic transport. Prog. Neurobiol. 2:205–255.Google Scholar
  2. 2.
    Miani, N., Di Girolamo, A., andDi Girolamo, M. 1966. Sedimentation characteristics of axonal RNA in rabbit. J. Neurochem. 13:755–759.PubMedGoogle Scholar
  3. 3.
    Amaldi, P., andRusca, G. 1970. Autoradiographic study of RNA on nerve fibers on embryonic sensory ganglion culturedin vitro under NGF stimulation. J. Neurochem. 17:767–771.PubMedGoogle Scholar
  4. 4.
    Austin, L., Bray, J. J., andYoung, R. J. 1966. Transport of protein and ribonucleic acid along nerve axons. J. Neurochem. 13:1267–1269.PubMedGoogle Scholar
  5. 5.
    Bondy, S. C. 1971. Axonal transport of macromolecules II. Nucleic acid migration in the central nervous system. Exp. Brain Res. 13:135–139.PubMedGoogle Scholar
  6. 6.
    Bray, J. J., andAustin, L. 1968. Flow of protein and RNA in peripheral nerve. J. Neurochem. 15:731–740.Google Scholar
  7. 7.
    Brink, J. J., andKarnovsky, M. L. 1973. Axoplasmic flow of exogenous RNA in the rat optic nerve. J. Neurochem. 21:1003–1007.PubMedGoogle Scholar
  8. 8.
    Ingoglia, N. A., Grafstein, B., McEwan, B. S., andMcQuarrie, I. G. 1973. Axonal transport of radioactivity in the goldfish optic system following intraocular injection of labelled RNA precursors. J. Neurochem. 20:1605–1615.PubMedGoogle Scholar
  9. 9.
    Autilio-Gambetti, L., Gambetti, P., andShafer, B. 1971. RNA and axonal flow. Biochemical and autoradiographic study in the rabbit optic system. Brain Res. 53:387–398.Google Scholar
  10. 10.
    Gambetti, P., Autilio-Gambetti, L., Shafer, B., andPfaff, L. 1973. Quantitative autoradiographic study of labelled RNA in rabbit optic nerve after intraocular injection of [3H]uridine. J. Cell Biol. 59:677–684.PubMedGoogle Scholar
  11. 11.
    Schubert, P., andKreutzberg, G. W. 1974. Axonal transport of adenosine and uridine derivatives and transfer to post-synaptic neurons. Brain Res. 76:526–530.PubMedGoogle Scholar
  12. 12.
    Gambetti, P., Ingoglia, N., Weis, P., andAutilio-Gambetti, L. 1975. RNA distribution in goldfish optic tectum after intraocular injection of [3H]uridine. An EM autoradiographic study during optic nerve regeneration. Abstr. 5th Ann. Meeting Soc. Neurosci. p. 797.Google Scholar
  13. 13.
    Peterson, J. A., Bray, J. J., andAustin, L. 1968. An autoradiographic study of the flow of protein and RNA along peripheral nerve. J. Neurochem. 15:741–745.Google Scholar
  14. 14.
    Bondy, S. C., andMadson, C. J. 1973. Axoplasmic transport of RNA. J. Neurobiol. 4:435–442.Google Scholar
  15. 15.
    Ingoglia, N. A., Sellin, L. C., andLinquist, T. D. 1976. The effect of cordecepin on the appearance of3H-RNA in the goldfish optic tectum following intraocular injection of3H-uridine. J. Neurochem. 27:179–184.PubMedGoogle Scholar
  16. 16.
    Ingoglia, N. A., andTuliszewski, T. 1976. Transfer RNA may be axonally transported during regeneration of goldfish optic nerves. Brain Res. 112:371–381.PubMedGoogle Scholar
  17. 17.
    Lasek, R. J., Dabrowski, C., andNorlander, R. 1973. Analysis of axoplasmic RNA from invertebrate giant axons. Nature (London) New Biol. 244:162–165.Google Scholar
  18. 18.
    Solymosy, F., Lazar, G., andBagi, G. 1970. An improved version of the diethyl pyrocarbonate method for extracting ribosomal nucleic acids. Anal. Biochem. 38:40–45.PubMedGoogle Scholar
  19. 19.
    Kirby, K. S. 1965. Isolation and characterization of ribosomal ribonucleic acid. Biochem. J. 96:266–269.PubMedGoogle Scholar
  20. 20.
    Ehrenberg, L., Fedorsak, I., andSolymosy, F. 1976. Diethyl pyrocarbonate in nucleic acid research. Prog Nucleic Acid Res. Mol. Biol. 16:189–255.PubMedGoogle Scholar
  21. 21.
    Loening, U. E. 1969. The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophoresis. The effects of changes in conformation. Biochem. J. 113:131–138.PubMedGoogle Scholar
  22. 22.
    Weinberg, R. A. 1973. Nuclear RNA metabolism. Annu. Rev. Biochem. 42:329–354.PubMedGoogle Scholar
  23. 23.
    Gray, E. G., andHamlyn, L. H. 1962. Electron microscopy of experimental degeneration in avian optic tectum. J. Anat. 96:309–316.Google Scholar
  24. 24.
    Carton, H. C., andAppel, S. H. 1973. The contribution of axoplasmic flow in optic nerve and protein synthesis within the optic tectum to synaptic membrane proteins of the chick optic tectum. J. Neurochem. 20:1707–1717.PubMedGoogle Scholar
  25. 25.
    Borek, E., andSrinivasan, P. R. 1966. The methylation of tRNA. Annu. Rev. Biochem. 35:275–298.Google Scholar
  26. 26.
    Purdy, J. L., Bondy, S. C., andBabitch, J. A. 1977. Axoplasmic migration of RNA containing a polyadenylic acid segment. Trans. Am. Soc. Neurochem. 8:256.Google Scholar
  27. 27.
    Soffer, R. L. 1974. Aminoacyl tRNA transferases. Adv. Enzymol. 40:90–139.Google Scholar

Copyright information

© Plenum Publishing Corporation 1978

Authors and Affiliations

  • Suzanne Por
    • 1
  • P. W. Gunning
    • 1
  • P. L. Jeffrey
    • 1
  • L. Austin
    • 1
  1. 1.Department of BiochemistryMonash UniversityClaytonAustralia

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