Advertisement

Neurochemical Research

, Volume 2, Issue 1, pp 59–85 | Cite as

Immunochemical characterization of synaptosomal membrane antigens from chicken brain

Histochemical localization in the day-old chick
  • John A. P. Rostas
  • Peter L. Jeffrey
Original Articles

Abstract

A set of synaptic membrane antigens has been investigated in a number of tissues by indirect immunofluorescence histochemistry, using an antiserum (SPM-I) raised against a purified synaptosomal plasma membrane fraction prepared from day-old chick forebrain. The antigens were found to be present in both the central and peripheral nervous systems and none of them were restricted to the forebrain. The antigens were not detectable in nonneural tissues except for the adrenal medulla. Since the antigens could not be detected in a number of clearly defined glial cell populations or a surgically induced gliosis of the optic nerve, the antigens appear to be nerve specific. The antigens were not present in all types of neurons, thus indicating that surface membrane differences exist between different classes of neurons. Within the plasma membrane of the nerve cell the antigens were not uniformly distributed: they were present in the synaptic region and, in some nerve cells, also in axonal region but were absent from perikaryal membranes and extended regions of dendritic membranes. In the sciatic nerve the antigens were transported at the fast rate of anterograde axonal transport as well as in the retrograde direction. These results have been compared with previous attempts to detect nerve-specific membrane components by immunological means.

Keywords

Sciatic Nerve Adrenal Medulla Axonal Region Synaptic Membrane Plasma Membrane Fraction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Aketser, A. R. 1971. The blood vascular system.In Physiology and Biochemistry of the Domestic Fowl, Vol. 2. D. J. Bell and B. M. Freeman (eds.), Academic Press, pp. 783–839.Google Scholar
  2. 2.
    Andres, K. H. 1970. Anatomy and ultrastructure of the olfactory bulb in fish, amphibia, reptiles, birds and mammals.In CIBA Foundation Symposium on Taste and Smell in Vertebrates. G. E. W. Wolstenholme and J. Knight (eds.), pp. 177–194.Google Scholar
  3. 3.
    Bignami, A., andDahl, D. 1973. Differentiation of astrocytes in the cerebellar cortex and pyramidal tracts of the newborn rat. An immunofluorescence study with antibodies to a protein specific to astrocytes.Brain Res. 49: 393–402.Google Scholar
  4. 4.
    Bock, E., andJorgensen, O. S. 1975. Rat brain synaptic vesicles and synaptic plasma membranes compared by crossed immunoelectrophoresis.FEBS Letters 52: 37–39.Google Scholar
  5. 5.
    Bock, E., Jorgensen, O. S., andMorris, S. J. 1974. Antigen-antibody crossed electrophoresis of rat brain synaptosomes and synaptic vesicles: correlation to watersoluble antigens from rat brain.J. Neurochem. 22: 1013–1017.Google Scholar
  6. 6.
    Boyd, I. A., andDavey, M. R. 1968. Composition of Peripheral Nerves. E. & S. Livingstone Ltd., Edinburgh and London.Google Scholar
  7. 7.
    Cantino, D., andMugnaini, E. 1974. Adrenergic innervation of the parasympathetic ciliary ganglion in the chick.Science 185: 279–281.Google Scholar
  8. 8.
    Cotman, C. W., andMatthews, D. A. 1971. Synaptic plasma membranes from rat brain synaptosomes: Isolation and partial characterization.Biochim. Biophys. Acta 249: 380–394.Google Scholar
  9. 9.
    Crossland, W. J., Cowan, W. M., Rogers, L. A., andKelly, J. P. 1974. The specification of the retino-tectal projection in the chick.J. Comp. Neur. 155: 127–164.Google Scholar
  10. 10.
    Dahlstrom, A. 1971. Axoplasmic transport (with particular respect to adrenergic neurons).Phil. Trans. R. Soc. Lond. B. 261: 325–358.Google Scholar
  11. 11.
    D'Amelio, W., Mutolo, V., andBarbarino, A. 1963. Immunological and electrophoretic analysis of rat liver mitochondria and other cellular fractions.Exp. Cell Res. 29: 1–16.Google Scholar
  12. 12.
    DeHeer, D. H., Olson, M. S., andPickard, R. N. 1974. Characterization of rat liver subcellular membranes: Demonstration of membrane-specific autoantigens.J. Cell Biol. 60: 460–472.Google Scholar
  13. 13.
    DeRobertis, E., Lapetina, E., Pecci Saavadra, J., andSoto, E. F. 1966,In vivo andin vitro action of antisera against isolated nerve endings of brain cortex.Life Sci. 5: 1979–1989.Google Scholar
  14. 14.
    Droz, B. 1973. Renewal of synaptic proteins.Brain Res. 62: 383–394.Google Scholar
  15. 15.
    Edds, M. V., Barkley, D. S., andFambrough, D. M. 1972. Genesis of neuronal patterns.Neurosci. Res. Prog. Bull., 10: 253–367.Google Scholar
  16. 16.
    Gottlieb, D. I., Merrell, R., andGlaser, L. 1974. Temporal changes in embryonal cell surface recognition.Proc. Natl. Acad. Sci. USA 71: 1800–1802.Google Scholar
  17. 17.
    Greengard, P., andKebabian, J. W. 1974. Role of cyclic AMP in synaptic transmission in the mammalian peripheral nervous system.Fed. Proc. 33: 1059–1067.Google Scholar
  18. 18.
    Hartman, B. K., Zide, D., andUdenfriend, S. 1972. The use of dopamine-β-hydroxylase as a marker for the central noradrenergic nervous system in the rat brain.Proc. Natl. Acad. Sci. USA 69: 2722–2726.Google Scholar
  19. 19.
    Herschman, H. R., Cotman, C. W., andMatthews, D. A. 1972. Serological specificities of brain subcellular organelles. I. Antisera to synaptosomal fractions.J. Immunol. 108: 1362–1369.Google Scholar
  20. 20.
    Hess, A. 1965. Developmental changes in the structure of the synapse on the myelinated cell bodies of the chicken ciliary ganglion.J. Cell Biol. 25: 1–19.Google Scholar
  21. 21.
    Hirose, G., andBass, N. H. 1974. A quantitative histochemical study of early cellular events associated with destruction of myelinated axons in rat optic nerve.Exp. Neurol. 44: 82–95.Google Scholar
  22. 22.
    Hubbel, W. L., andMcConnell, H. M. 1968. Spin label studies of the excitable membranes of nerve and muscle.Proc. Natl. Acad. Sci. USA 61: 12–16.Google Scholar
  23. 23.
    Huber, G. C., andCrosby, E. C. 1929. The nuclei and fibre paths of the avian diencephalon, with consideration of telencephalic and certain mesencephalic centers and connections.J. Comp. Neur. 48: 1–226Google Scholar
  24. 24.
    Hunt, R. K., andJacobson, M. 1973. Specification of positional information in retinal ganglion cells ofXenopus: assays for analysis of the unspecified state.Proc. Natl. Acad. Sci. USA 70: 507–511.Google Scholar
  25. 25.
    Jarosch, E., andPrecht, W. 1972. Effects of antibodies directed towards membrane fragments of synaptosomes on cerebellar field potentials.Brain Res. 42: 225–229.Google Scholar
  26. 26.
    Jeffrey, P. L., andGunning, P. W. 1976. The nature and location of chick synaptosomal plasma membrane RNA. Submitted to Biochem. Biophys. Acta.Google Scholar
  27. 27.
    Johnson Schneider, D. 1973. Studies of nervous system protein.In Proteins of the Nervous System, D. Johnson Schneider (ed.), Raven Press, pp. 67–94.Google Scholar
  28. 28.
    Kawamura, A. 1969. Fluorescent antibody techniques and their applications. Manchester, University Park Press.Google Scholar
  29. 29.
    Kleinschuster, S. J., andMoscona, A. A. 1972. Interactions of embryonic and fetal neural cells with carbohydrate-binding phytoagglutinins: Cell surface changes with differentiation.Exp. Cell Res. 70: 397–410.Google Scholar
  30. 30.
    Kornguth, S. E., Anderson, J. W., andScott, G. 1969. Isolation of synaptic complexes in a caseium chloride density gradient: Electronmicroscopic and immunohistochemical studies.J. Neurochem. 16: 1017–1024.Google Scholar
  31. 31.
    Kuhns, W. J., Weinbaum, G., Turner, R., andBurger, M. M. 1974. Sponge aggregation: A model for studies on cell-cell interactions.Ann. N.Y. Acad. Sci. 234: 58–74.Google Scholar
  32. 32.
    Landmesser, L., andPilar, G. 1970. Selective reinnervation of two cell populations in the adult pigeon ciliary ganglion.J. Physiol. 211: 203–216.Google Scholar
  33. 33.
    Lim, R., andHsu, L. W. 1971. Studies on brain specific membrane proteins.Biochim. Biophys. Acta 249: 569–582.Google Scholar
  34. 34.
    Livett, B. G., Rostas, J. A. P., Jeffrey, P. L., andAustin, L. 1974. Antigenicity of isolated synaptosomal membranes.Exp. Neurol. 43: 330–338.Google Scholar
  35. 35.
    Livett, B. G., Howe, P. R. C., Fenwick, E. M., andAustin, L. 1975. An immunochemical approach to the transport of axonal vesicular proteins and their release from nerve terminals.In Recent Advances in Myology. W. G. Bradley, D. Gardner-Meduin, and J. N. Walton (eds.), Excerpta Medica.Google Scholar
  36. 36.
    Lundkirst, U., Perlmann, P., andGoeringer, G. C. 1964. Antigens in microsomes and other subcellular fractions of parenchymal and reticuloendothelial cells of rat liver: Fluorescent antibody staining.In Protides of the Biological Fluids, H. Peeters (ed.), Vol. 12, Elsevier, Amsterdam, pp 236–241.Google Scholar
  37. 37.
    Macpherson, C. F., Shek, R. P., andPay, P. 1973. Immunochemical analysis of rat brain microsomes.Immunochemistry 10: 409–415.Google Scholar
  38. 38.
    McClay, D. R., andMoscona, A. A. 1974. Purification of the specific cellaggregating factor from embryonic neural retina cells.Exp. Cell Res. 87: 438–443.Google Scholar
  39. 39.
    Matus, A. I. 1975. Immunohistochemical demonstration of antigen associated with the post-synaptic lattice.J. Neurocytol. 4: 55–62.Google Scholar
  40. 40.
    Mickey, D. D., McMillan, P. N., Appel, S. H., andDay, E. D. 1971. Specificity and cross-reactivity of antisynaptosome antibodies as determined by sequential absorption analysis.J. Immunol. 107: 1599–1610.Google Scholar
  41. 41.
    Nairn, R. C. 1969. Fluorescent Protein Tracing. E. & S. Livingstone Ltd., Edinburgh and London.Google Scholar
  42. 42.
    Nicolson, G. L., andSinger, S. J., 1974. The distribution and asymmetry of mammalian cell surface saccharides utilizing ferritin-conjugated plant agglutinins as specific saccharide stains.J. Cell Biol. 60: 236–248.Google Scholar
  43. 43.
    Orosz, A., Hamori, J., Falus, A., Madarasz, E., Lakos, I., andAdam, G. 1973. Specific antibody-fragments against the post-synaptic web.Nature New Biol. 245: 18–19.Google Scholar
  44. 44.
    Orosz, A., Madarasz, E., Falus, A., andAdam, G. 1974. Demonstration of detergent-soluble antigen specific for the synaptosomal membrane-fraction isolated from the cat cerebral cortex.Brain Res. 76: 119–131.Google Scholar
  45. 45.
    Perlmann, P., Goeringer, G. C., andLundkirst, U. 1964. Antigens in microsomes and other subcellular fractions of parenchymal and reticuloendothelial cells of rat liver: Agar diffusion studies.In Protides of the Biological Fluids. H. Peeters (ed.), Vol. 12, Elsevier, Amsterdam, pp. 232–235.Google Scholar
  46. 46.
    Raiteri, M., andBertolini, A. 1974. Specificity and cross-reactivity of antisynaptosome antibodies.Brain Res. 65: 297–302.Google Scholar
  47. 47.
    Raiteri, M., Bertolini, A., andLaBella, R. 1972. Synaptosome antisera affect permeability of synaptosomal membranesin vitro.Nature New Biol. 238: 242–243.Google Scholar
  48. 48.
    Ramon y Cajal, S. 1911. Histologie du systeme nerveaux de l'Homme et des Vertebres 2. Paris: A. Maloine.Google Scholar
  49. 49.
    Reese, T. S., andShepherd, G. M. 1972. Dendro-dendritic synapse in the central nervous system.In Structure and Function of Synapses. G. D. Pappas and D. P. Purpura (eds.), Raven Press, pp. 121–136.Google Scholar
  50. 50.
    Rostas, J. A. P., andJeffrey, P. L. 1975. Restricted mobility of neuronal membrane antigens.Neurosci. Lett. 1: 47–53.Google Scholar
  51. 51.
    Rostas, J. A. P., andJeffrey, P. L. 1977. Immunochemical characterization of synaptosomal membrane antigens from chicken. Histochemical demonstration of changes in distribution during development.Neurochemical Res. In press.Google Scholar
  52. 52.
    Rostas, J. A. P., andJeffrey, P. L. 1977. Immunochemical characterization of synaptosomal membrane antigens from chicken. Quantitative determination and partial characterization.Neurochemical Res. In press.Google Scholar
  53. 53.
    Roth, S. 1973. A molecular model for cell interactions.Q. Rev. Biol. 48: 541–563.Google Scholar
  54. 54.
    Springer, G. F., Desai, P. R., andBanatwala, I. 1974. Blood group MN specific substances and precursors in normal and malignant human breast tissues.Naturwissenschaften 61: 457–458.Google Scholar
  55. 55.
    Wells, J. W., andWright, P. A. L. 1971. The adrenal glands.In Physiology and Biochemistry of the Domestic Fowl, Vol. 1, D. J. Bell and B. M. Freeman (eds.), Academic Press, pp. 489–520.Google Scholar
  56. 56.
    Wenzel, B. M. 1973. Chemoreception.In Avian Biology, Vol. III. D. S. Farner and J. R. King (eds.), Academic Press, pp. 389–415.Google Scholar
  57. 57.
    Whitbeck, E. G., andRosenberg, L. T. 1964. Antigenic properties of microsomes from guinea-pig spleen, liver and lymph modes.Immunology 7: 363–374.Google Scholar

Copyright information

© Plenum Publishing Corporation 1977

Authors and Affiliations

  • John A. P. Rostas
    • 1
  • Peter L. Jeffrey
    • 1
  1. 1.Department of BiochemistryMonash UniversityClaytonAustralia

Personalised recommendations