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Neurochemical Research

, Volume 2, Issue 1, pp 51–57 | Cite as

Synthesis of affinity chromatography resins for the purification of brain glutamate decarboxylase

  • Lourival D. Possani
  • Alejandro Bayón
  • Ricardo Tapia
Original Articles

Abstract

In the present paper we describe the synthesis of Sepharose-boundN-(5′-phosphopyridoxyl)-amino-oxyacetic acid,N-(5′-phosphopyridoxyl)-canaline, andN-(5′-phosphopyridoxyl)-α,γ-diaminobutyric acid (Seph-DAB-PLP), designed for binding specifically brain glutamate decarboxylase (GAD). The three Sepharose-N-(phosphopyridoxyl)-amino acids were capable of binding GAD from a mouse brain soluble preparation. The enzyme can be purified up to 25-fold in one step by affinity chromatography on Seph-DAB-PLP.

Keywords

Glutamate Mouse Brain Affinity Chromatography Canaline Glutamate Decarboxylase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1977

Authors and Affiliations

  • Lourival D. Possani
    • 1
  • Alejandro Bayón
    • 1
  • Ricardo Tapia
    • 1
  1. 1.Departamento de Biología Experimental, Instituto de BiologíaUniversidad Nacional Autónoma de MéxicoMéxico 20México

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