Neurochemical Research

, Volume 2, Issue 1, pp 51–57 | Cite as

Synthesis of affinity chromatography resins for the purification of brain glutamate decarboxylase

  • Lourival D. Possani
  • Alejandro Bayón
  • Ricardo Tapia
Original Articles


In the present paper we describe the synthesis of Sepharose-boundN-(5′-phosphopyridoxyl)-amino-oxyacetic acid,N-(5′-phosphopyridoxyl)-canaline, andN-(5′-phosphopyridoxyl)-α,γ-diaminobutyric acid (Seph-DAB-PLP), designed for binding specifically brain glutamate decarboxylase (GAD). The three Sepharose-N-(phosphopyridoxyl)-amino acids were capable of binding GAD from a mouse brain soluble preparation. The enzyme can be purified up to 25-fold in one step by affinity chromatography on Seph-DAB-PLP.


Glutamate Mouse Brain Affinity Chromatography Canaline Glutamate Decarboxylase 
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Copyright information

© Plenum Publishing Corporation 1977

Authors and Affiliations

  • Lourival D. Possani
    • 1
  • Alejandro Bayón
    • 1
  • Ricardo Tapia
    • 1
  1. 1.Departamento de Biología Experimental, Instituto de BiologíaUniversidad Nacional Autónoma de MéxicoMéxico 20México

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