Neurochemical Research

, Volume 11, Issue 7, pp 983–996 | Cite as

Ultrastructure of reconstituted membranes containing the muscarinic receptor

  • Amanda Pellegrino de Iraldi
  • José Santiago Aguilar
  • Enrique L. M. Ochoa
Original Articles
Accepted:

Abstract

In this paper the demonstration is made that membrane vesicles (liposomes) containing the muscarinic receptor can be formed by polyethylene glycol (PEG) precipitation of detergent extracts of bovine atrial membranes. The incorporation of the muscarinic receptor in these vesicles may be related to the restoration of the heterogeneity and nucleotide modulation of muscarinic agonist binding by PEG precipitation of atrial detergent extracts, previously reported. Vesicles are also formed when detergent solubilized asolectin lipids, alone or in combination with membrane detergent extracts, are precipitated by PEG. The structure of the vesicles seems depend on their lipid and protein composition and the procedure employed for the removal of the dispersing medium. These results indicate that PEG precipitation could be used for the reconstitution of the muscarinic receptor into the liposomes of exogenous lipids.

Keywords

Lipid Nucleotide Polyethylene Glycol Muscarinic Receptor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Plenum Publishing Corporation 1986

Authors and Affiliations

  • Amanda Pellegrino de Iraldi
    • 1
  • José Santiago Aguilar
    • 1
  • Enrique L. M. Ochoa
    • 1
  1. 1.Facultad de MedicinaInstituto de Biología CelularBuenos AiresArgentina

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