Neurochemical Research

, Volume 16, Issue 6, pp 675–679 | Cite as

Expression of actin and myosin genes during PC12 cell differentiation

  • R. C. Henke
  • O. Tolhurst
  • J. W. Sentry
  • P. Gunning
  • P. L. Jeffrey
Original Articles


We have measured the accumulation of transcripts for myosin and actin during NGF induced differentiation of PC12 cells. Beta (β) and gamma (γ) actin and myosin light chains (MLC) 2 and 3 show different patterns of expression, with transient elevations in gene expression one day after NGF addition. This elevation occurs earlier than that of neurite outgrowth, neurofilament protein (NF68) (16) and Thy-1 glycoprotein gene expression. These results suggest differing mechanisms of control of actin and myosin expression, together with a varying function and relationship between them during NGF- induced neurite differentiation.


Gene Expression Cell Differentiation PC12 Cell Light Chain Neurite Outgrowth 
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Copyright information

© Plenum Publishing Corporation 1991

Authors and Affiliations

  • R. C. Henke
    • 1
  • O. Tolhurst
    • 1
  • J. W. Sentry
    • 1
  • P. Gunning
    • 1
  • P. L. Jeffrey
    • 1
  1. 1.Departments of Muscle Genetics and Developmental NeurobiologyChildren's Medical Research FoundationCamperdownAustralia

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