Neurochemical Research

, Volume 10, Issue 5, pp 591–599

The uptake of serotonin and dopamine by homogenates of frozen rat and human brain tissue

  • Anders Stenström
  • Lars Oreland
  • John Hardy
  • Per Wester
  • Bengt Winblad
Original Articles


A recently described procedure of freezing and thawing, which allows retention of metabolic and functional integrity, has been applied in the study of serotonin and dopamine uptake into frozen rat and post mortem human frozen tissue. TheKm andVmax for the serotonin uptake into human hypothalamus were estimated to be 0.12 μM and 0.03 nmol/g/min respectively. TheKm andVmax for the dopamine uptake into human putamen were estimated to be 0.28 μM and 0.13 nmol/g/min respectively. The results indicate that the freezing procedure does not affect the uptake sites for these transmitters. The storage time before freezing is however of importance for theVmax value. TheKm value for the uptake, on the other hand, seems to be rather resistant to storage time before freezing.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Horn, A. S. 1979. The characteristics of dopamine uptake. Pages 217–235,in Horn, A. S., Korf, J., andWesterink, B. M. C. (eds.), Neurobiology of Dopamine, Academic Press, London.Google Scholar
  2. 2.
    Ross, S. B. 1982. The characteristics of serotonin uptake systems. Pages 159–195,in Osborne, N. N. (ed.), The Biology of Serotonergic Transmission, John Wiley & Sons Ltd., London.Google Scholar
  3. 3.
    Olson, L., Nyström, B., andSeiger, Å. 1973. Monoamine fluorescence histochemistry of human post mortem brain. Brain Res. 63:231–247.Google Scholar
  4. 4.
    Garey, R. E., andHeath, R. G. 1974. Uptake of catecholamines by human synaptosomes. Brain Res. 79:520–523.Google Scholar
  5. 5.
    Hardy, J. A., andDodd, P. R. 1983. Metabolic and functional studies on post mortem human brain. Neurochem. Internat. 5:253–266.Google Scholar
  6. 6.
    Weiner, N., Martin, C., Wesemann, W., andRiederer, P. 1979. Effect of post mortem storage on synaptic 5-HT binding and uptake in mammalian brain. J. Neural. Transm. 46:253–262.Google Scholar
  7. 7.
    Schwarcz, R. 1981. Effects of tissue storage and freezing on brain glutamate uptake. Life Sci. 28:1147–1154.Google Scholar
  8. 8.
    Haan, E. A., andBowen, D. M. 1981. Protection of neocortical tissue prisms from freeze-thaw injury by dimethyl sulfoxide. J. Neurochem. 37:243–246.Google Scholar
  9. 9.
    Hardy, J. A., Dodd, P. R., Oakley, A. E., Perry, R. H., Edwardson, J. A., andKidd, A. M. 1983. Metabolically active synaptosomes can be prepared from frozen rat and human brain. J. Neurochem. 40:608–614.Google Scholar
  10. 10.
    Winblad, B. 1980. Post mortem dissection of human brain and spinal cord. Pages 226–240,in Pope, A. (ed.), Human Brain Dissection. USA Dept. of Health and Human Services, Washington.Google Scholar
  11. 11.
    Paton, D. M. 1980. Neuronal transport of noradrenaline and dopamine. Pharmacol. 21:85–92.Google Scholar
  12. 12.
    Lloyd, K. G. 1972. Ph.D. thesis, Dept. of Pharmacology, University of Toronto: “Biogenic amines and related enzymes in the human and animal brain”.Google Scholar
  13. 13.
    Cheetham, S. C., Dodd, R. R., Edwardson, J. A., Hardy, J. A., andKidd, A. M. 1983. Kinetic characteristics of GABA uptake by synaptosomes performed from frozen post mortem human brain. Brit. J. Pharm. 79:267.Google Scholar
  14. 14.
    Holzbauer, M., andSharman, D. F. 1972. The distribution of catecholamines in vertebrates. Pages 148,in Blaschko, H., andMuscholl, E. (eds.), Handbook of Experimental Pharmacology, Vol. XXXIII, Springer Verlag, Berlin.Google Scholar

Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • Anders Stenström
    • 1
  • Lars Oreland
    • 2
  • John Hardy
    • 3
  • Per Wester
    • 3
  • Bengt Winblad
    • 3
  1. 1.Department of PharmacologyUniversity of UmeåUmeåSweden
  2. 2.Department of PharmacologyUniversity of GothenburgGothenburgSweden
  3. 3.Department of PathologyUniversity of UmeåUmeåSweden

Personalised recommendations