Neurochemical Research

, Volume 10, Issue 5, pp 591–599

The uptake of serotonin and dopamine by homogenates of frozen rat and human brain tissue

  • Anders Stenström
  • Lars Oreland
  • John Hardy
  • Per Wester
  • Bengt Winblad
Original Articles

Abstract

A recently described procedure of freezing and thawing, which allows retention of metabolic and functional integrity, has been applied in the study of serotonin and dopamine uptake into frozen rat and post mortem human frozen tissue. TheKm andVmax for the serotonin uptake into human hypothalamus were estimated to be 0.12 μM and 0.03 nmol/g/min respectively. TheKm andVmax for the dopamine uptake into human putamen were estimated to be 0.28 μM and 0.13 nmol/g/min respectively. The results indicate that the freezing procedure does not affect the uptake sites for these transmitters. The storage time before freezing is however of importance for theVmax value. TheKm value for the uptake, on the other hand, seems to be rather resistant to storage time before freezing.

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Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • Anders Stenström
    • 1
  • Lars Oreland
    • 2
  • John Hardy
    • 3
  • Per Wester
    • 3
  • Bengt Winblad
    • 3
  1. 1.Department of PharmacologyUniversity of UmeåUmeåSweden
  2. 2.Department of PharmacologyUniversity of GothenburgGothenburgSweden
  3. 3.Department of PathologyUniversity of UmeåUmeåSweden

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