Neurochemical Research

, Volume 6, Issue 12, pp 1309–1317 | Cite as

Elevation of cerebral levels of nonessential amino acids in vivo by administration of large doses

  • Eugene Toth
  • Abel Lajtha
Original Articles


Taurine, aspartic acid, glutamic acid, glycine, and GABA were administered either intragastrically or in liquid diets to mice and rats. This resulted in a great increase in the plasma concentration of the administered amino acid, with plasma levels remaining elevated for several days.

The prolonged increase in plasma levels resulted in significant increases in brain levels. Under these experimental conditions, taurine, aspartic acid, and glutamic acid were increased 30–60%; glycine and GABA 100%. During these experiments, plasma levels of taurine, aspartate, and glutamate were below brain levels; those of glycine and GABA were above.

The findings show that even slowly penetrating amino acid levels can be increased in brain after parenteral administration of large doses.


Aspartate Glutamate Plasma Concentration Glycine Plasma Level 
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  1. 1.
    Lajtha, A., andToth, J. 1961. The brain barrier system. II. Uptake and transport of amino acids by the brain. J. Neurochem. 8:216–225.PubMedGoogle Scholar
  2. 2.
    Battistin, L., Grynbaum, A., andLajtha, A. 1971. The uptake of various amino acids by the mouse brain in vivo. Brain Res. 29:85–99.PubMedGoogle Scholar
  3. 3.
    Schwerin, P., Bessman, S. P., andWaelsch, H. 1950. The uptake of glutamic acid and glutamine by brain and other tissues of the rat and mouse. J. Biol. Chem. 184:37–44.PubMedGoogle Scholar
  4. 4.
    van Gelder, N. M., andElliott, K. A. C. 1958. Disposition of γ-aminobutyric acid administered to mammals. J. Neurochem. 3:139–143.PubMedGoogle Scholar
  5. 5.
    Oldendorf, W. H., andSzabo, J. 1976. Amino acid assignment to one of three blood-brain barrier amino acid carriers. Amer. J. Physiol. 230:94–98.PubMedGoogle Scholar
  6. 6.
    Toth, J., andLajtha, A. 1977. Rates of exchange of free amino acids between plasma and brain in mice. Neurochem. Res. 2:149–160.Google Scholar
  7. 7.
    Pratt, O. E. 1976. The transport of metabolizable substances into the living brain. Pages 55–75,in Levi, G., Battistin, L., andLajtha, A., (eds.), Transport Phenomena in the Nervous System: Physiological and Pathological Aspects, Vol. 69, Advances in Experimental Medicine and Biology, Plenum, New York.Google Scholar
  8. 8.
    van Gelder, N. M., Sherwin, A. L., andRasmussen, T. 1972. Amino acid content of epileptogenic human brain: focal versus surrounding regions. Brain Res. 40:385–393.PubMedGoogle Scholar
  9. 9.
    Perry, T. L., Hansen, S., andKloster, M. 1973. Huntington's Chorea: Deficiency of γ-aminobutyric acid in brain. N. Engl. J. Med. 288:337–342.PubMedGoogle Scholar
  10. 10.
    Toth, J., andLajtha, A. 1980. Effect of protein-free diet on the uptake of amino acids by the brain in vivo. Exp. Neurol. 68:443–452.PubMedGoogle Scholar
  11. 11.
    Oldendorf, W. H. 1977. The blood-brain barrier. Exp. Eye Res. Suppl. 177–190.Google Scholar
  12. 12.
    Liebshultz, J., Airoldi, I., Brownstein, M. J., Chinn, N. G., andWurtman, R. J. 1977. Regional distribution of endogenous and parenteral glutamate, aspartate and glutamine in rat brain. Biochem. Pharmacol. 26:443–446.PubMedGoogle Scholar
  13. 13.
    Sturman, J. A. 1973. Taurine pool size in the rat: effects of vitamin B6 deficiency and high taurine diet. J. Nutr. 103:1566–1580.PubMedGoogle Scholar
  14. 14.
    Perry, T. L., andHansen, S. 1973. Sustained drug-induced elevation of brain GABA in the rat. J. Neurochem. 21:1167–1175.PubMedGoogle Scholar
  15. 15.
    Roberts, E., andKuriyama, K. 1968. Biochemical-physiological correlations in studies of the γ-aminobutyric acid system. Brain Res. 8:1–35.PubMedGoogle Scholar
  16. 16.
    Kandera, J., Levi, G., andLajtha, A. 1968. Control of cerebral metabolite levels—II. Amino acid uptake and levels in various areas of the rat brain. Arch. Biochem. Biophys. 126:249–260.PubMedGoogle Scholar
  17. 17.
    Hrsuska, R. E., Thut, P. D., Huxtable, R., andBressler, R. 1976. Hypothermia produced by taurine. Pages 347–356,in Huxtable andBarbeau, A. (eds.), Taurine, Raven Press, New York.Google Scholar
  18. 18.
    Joseph, M. H., andEmson, P. C. 1976. Taurine and cobalt induced epilepsy in the rat: biochemical and electrocorticographic study. J. Neurochem. 27:1495–1501.PubMedGoogle Scholar
  19. 19.
    van Gelder, N. H. 1972. Antagonism by taurine of cobalt induced epilepsy in cat and mouse. Brain Res. 47:157–165.PubMedGoogle Scholar
  20. 20.
    Maher, T., andWurtman, R. J. 1980. L-Threonine administration increased glycine concentrations in the rat central nervous system. Life Sci. 26:1283–1286.PubMedGoogle Scholar
  21. 21.
    Fletcher, A., andFowler, L. J. 1980. γ-Aminobutyric acid metabolism in rat brain following chronic oral administration of ethanolamine O-sulphate. Biochem. Pharmacol. 29:1451–1454.PubMedGoogle Scholar
  22. 22.
    Tell, G., Bohlen, P., Schechter, P. J., Koch-Weser, J., Agid, Y., Bonnet, G., Coquillat, G., Chazot, G. andFischer, C. 1981. Treatment of Huntington disease with γ-acetylenic GABA, an irreversible inhibitor of GABA-transaminase: increased CSF GABA and homocarnosine without clinical amelioration. Neurology 31:207–211.PubMedGoogle Scholar
  23. 23.
    Grove, J., Schechter, P. J., Tell, G., Koch-Weser, J., Sjoerdsma, A., Water, J. M., Marescaux, C., andRumbach, L. 1981. Increase gamma-aminobutyric acid (GABA), homocarnosine and β-alanine in cerebrospinal fluid of patients treated with γ-vinyl GABA (4-amino-hex-5-enoic acid). Life Sci. 28:2431–2439.PubMedGoogle Scholar
  24. 24.
    van Gelder, N. M., Sherwin, A. L., Sacks, C., andAndermann, F. 1975. Biochemical observations following administration of taurine to patients with epilepsy. Brain Res. 94:297–306.PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1981

Authors and Affiliations

  • Eugene Toth
    • 1
  • Abel Lajtha
    • 1
  1. 1.Center For NeurochemistryRockland Research Institute Ward's IslandNew York

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