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Neurochemical Research

, Volume 9, Issue 2, pp 219–224 | Cite as

Inhibition by N-acetyl-aspartate of aspartate binding to a proteolipid fraction from rat cerebral cortex

  • M. Burgal
  • J. Lizondo
  • A. Jordá
  • S. Grisolía
Original Articles

Abstract

Many roles have been suggested for N-acetyl-aspartate in brain function because of it being located almost exclusively in that organ. However, its true role remains to be demonstrated. We show here that N-acetyl-aspartate: 1) binds to a hydrophobic protein fraction from the cerebral cortex of the rat, which specifically bindsl-aspartate,l-glutamate, and γ-amino-butyric acid; and 2) has a marked inhibitory effect on the aspartate binding sites of this proteolipid fraction. Structural analogs of N-acetyl-asparate, i.e. N-carbamyl-aspartate and N-methyl-aspartate also inhibit thel-aspartate binding by the brain protein fraction used.

Keywords

Aspartate Binding Site Cerebral Cortex Brain Function Protein Fraction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Tallan, H. H., Moore, S., andStein, W. H. 1956. N-acetyl-L-aspartic acid in brain. J. Biol. Chem. 219:257–264.PubMedGoogle Scholar
  2. 2.
    Curatolo, A., D'Arcangelo, P., Lino, A., andBrancati, A. 1965. Distribution of N-acetyl-aspartic and N-acetyl-aspartyl-glutamic acids in nervous tissue. J. Neurochem. 12:339–342.PubMedGoogle Scholar
  3. 3.
    Reichelt, K. L., andFonnum, F. 1969. Subcellular localization of N-acetyl-aspartyl-glutamate, N-acetyl-glutamate and glutathione in brain. J. Neurochem. 16:1409–1416.PubMedGoogle Scholar
  4. 4.
    Berlinguet, L., andLaliberté, M. 1970. Biosynthesis of N-acetyl-L-aspartic acid “in vivo” and in brain homogenates. Can. J. Biochem. 48:207–211.PubMedGoogle Scholar
  5. 5.
    Gaitonde, M. K. 1979. Decreased turnover of N-acetylaspartate in the brain of thiaminedeficient rats. J. Neurochem. 32:1603–1604.PubMedGoogle Scholar
  6. 6.
    Stevenard, M., andAiache, J. M. 1966. L'acide N-acetyl aspartique. Prod. et Prob. Pharm. 21:318–321.Google Scholar
  7. 7.
    Usherwood, P. N. R. 1978. Amino acids as neurotransmitters. Adv. Comp. Physiol. Biochem. 7:227–309.PubMedGoogle Scholar
  8. 8.
    Cecchi, L., DeSantis, A., Eusebi, F., andCuratolo, A. 1978. An electrophysiological study of N-acetyl-l-aspartic acid on the stellate ganglion of the squid. Experienta 34:1475–1476.Google Scholar
  9. 9.
    Curtis, D. R., andWatkins, J. C. 1960. The excitation and depression of spinal neurones by structurally related amino acids. J. Neurochem. 6:117–141.PubMedGoogle Scholar
  10. 10.
    Moore, S., andStein, W. H. 1954. Procedures for the chromatographic determination of amino acids on four per cent cross-linked sulfonated polystyrene resins. J. Biol. Chem. 211:893–906.PubMedGoogle Scholar
  11. 11.
    Abbott, D., andAndrews, R. S. 1970. Pages 26–48, in: An Introduction to Chromatography, 2nd Ed., Longmans, Green and Co, Ltd., London.Google Scholar
  12. 12.
    Nordmann, R., Gauchery, O., Ruisseau, J. P., Thomas, Y., andNordmann, J. 1954. Chromatographie sur papier des acides organiques non volatils des liquides biologiques. Bull. Sté. Chim. Biol. 36:1461–1471.Google Scholar
  13. 13.
    Hunninghake, D., andGrisolía, S. 1966. A sensitive and convenient micromethod for estimation of urea, citrulline and carbamyl derivatives. Anal. Biochem. 16:200–205.PubMedGoogle Scholar
  14. 14.
    Folch, J., andLees, M. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226:497–509.PubMedGoogle Scholar
  15. 15.
    De Robertis, E., andFiszer de Plazas, S. 1976. Differentiation ofl-aspartate and L-glutamate high-affinity binding sites in a protein fraction isolated from rat cerebral cortex. Nature 260:347–349.PubMedGoogle Scholar
  16. 16.
    Marshall, M., Metzenberg, R. L., andCohen, P. P. 1961. J. Biol. Chem. 236:2229. Cited inShigesada, K., Tatibana, M. 1971. J. Biol. Chem. 246:5588–5595.Google Scholar
  17. 17.
    De Robertis, E., andFiszer de Plazas, S. 1976. Isolation of hydrophobic proteins binding amino acids. Stereoselectivity of the binding ofl-14C-glutamic acid in cerebral cortex. J. Neurochem. 26:1237–1243.PubMedGoogle Scholar
  18. 18.
    Fiszer de Plazas, S., andDe Robertis, E. 1976. Isolation of hydrophobic proteins binding amino acids: L-Aspartic acid-binding protein from the rat cerebral cortex. J. Neurochem. 27:889–894.PubMedGoogle Scholar
  19. 19.
    Roux, H., Murthy, M. R. V., andBerlinguet, L. 1974. Role of N-acetyl-amino acids in cerebral protein synthesis. I. incorporation of radioactivity from labelled acetyl and amino-acyl moieties into protein. Brain Res. 79:253–265.PubMedGoogle Scholar
  20. 20.
    Knudsen, R., Lam, Y. K., Folkers, K., Frick, W., Caves, G. D. Jr., andBarofsky, D. F. 1978. Isolation of N-acetyl-aspartic acid from hypothalamic tissue and significance of its ACTH-releasing activity. Biochem. Biophys. Res. Commun. 80:735–739.PubMedGoogle Scholar
  21. 21.
    Curatolo, A., Cecchi, L., Eusebi, F., andDe Santis, A. 1979. N-acetyl-l-aspartic acid in the stellate ganglion of the squid. J. Neurochem. 32:1349–1350.PubMedGoogle Scholar
  22. 22.
    Burgal, M., Jordá, A., andGrisolía, S. 1982. Effects of N-acetyl aspartate, aspartate and glutamate on cAMP and cGMP levels in developing rat cerebral cortex. J. Neurochem. 38:1498–1500.PubMedGoogle Scholar
  23. 23.
    Michaelis, E. K. 1975. Partial purification and characterization of a glutamate-binding membrane glycoprotein from rat brain. Biochem. Biophys. Res. Commun. 65:1004–1012.PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • M. Burgal
    • 1
  • J. Lizondo
    • 1
  • A. Jordá
    • 1
  • S. Grisolía
    • 1
  1. 1.Instituto de Investigaciones Citológicas de la Caja de Ahorros de ValenciaValenciaSpain

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